吲哚美辛在体外可诱导大鼠子宫收缩，并改变其对钙和乙酰胆碱的反应性。

Indomethacin induces rat uterine contractions in vitro and alters reactivity to calcium and acetylcholine.

作者信息

Hargrove J L, Nesbitt D, Gaspar M J, Ellis L C

出版信息

Am J Obstet Gynecol. 1976 Jan 1;124(1):25-9. doi: 10.1016/0002-9378(76)90006-5.

Abstract

The initial contractions of uteri in vitro from castrated, estrogen-treated rats, were markedly diminished following replacement with fresh bathing medium. Indomethacin and aspirin (10(-5) to 10(-4)M) strongly stimulated such quiescent preparations and reduced their subsequent responsiveness to Ca++. Reintroducing the initial bathing medium (which contained prostaglandin-like material), or adding prostaglandin F2alpha to the fresh medium, initiated uterine contractions and restored responsiveness to calcium ion. Injections of indomethacin into castrated, estrogen-treated rats reduced initial in vitro uterine motility, abolished production of prostaglandin-like compounds, and prevented either indomethacin, aspirin, or Ca++ from stimulating uterine contractions. Uterine responsiveness to acetylcholine in vitro was significantly reduced in rats pretreated with indomethacin.

摘要

在体外，去势并用雌激素处理的大鼠子宫的初始收缩，在用新鲜的浴液替换后明显减弱。吲哚美辛和阿司匹林（10⁻⁵至10⁻⁴M）强烈刺激这种静止的制剂，并降低其随后对钙离子的反应性。重新引入初始浴液（其中含有类前列腺素物质），或向新鲜培养基中添加前列腺素F2α，可引发子宫收缩并恢复对钙离子的反应性。向去势并用雌激素处理的大鼠注射吲哚美辛可降低体外子宫的初始运动性，消除类前列腺素化合物的产生，并阻止吲哚美辛、阿司匹林或钙离子刺激子宫收缩。用吲哚美辛预处理的大鼠，其子宫对乙酰胆碱的体外反应性显著降低。