Cho Eun Gi, Noor Normah M, Kim Haeng Hoon, Rao V Ramanatha, Engelmann Florent
International Plant Genetic Resources Institute (IPGRI), Regional Office for Asia, Pacific and Oceania, P.O. Box 236, UPM Post Office, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
Cryo Letters. 2002 Sep-Oct;23(5):309-16.
The desiccation and freezing tolerance of seeds, with and without testas, and embryonic axes of Citrus aurantifolia were investigated. Seeds were desiccated with silica gel, under the laminar air flow cabinet or by placing them on a laboratory bench. Whatever the desiccation method employed, survival before and after cryopreservation was higher for seeds without testas. When freezing intact seeds, the highest survival percentage (41.3 %) was achieved after desiccation to 7.3 % moisture content (fresh weight basis) on the laboratory bench. Survival of seeds cryopreserved without testas could reach up to 85 % after desiccation under the laminar air flow cabinet or on the laboratory bench, corresponding to moisture contents of 7.1 and 4.5 %, respectively. After desiccation with silica gel, survival reached a maximum of 60.0 %, for a seed moisture content of 3.3 %. Survival of control embryonic axes was high (80-100 %) whatever the sucrose concentration in the preculture medium and the duration of the desiccation period. After cryopreservation, no survival was noted with embryonic axes, which had not been precultured nor desiccated. Survival of non-desiccated embryonic axes after cryopreservation increased progressively in line with increasing sucrose concentrations in the preculture medium, from 7.5 % with 0.1 M sucrose to 77.5 % with 0.7 M sucrose. Survival of desiccated and cryopreserved embryos was always high, whatever the preculture treatment and desiccation period, ranging from 55.8 % to 92.5 %.
对有无种皮的酸橙种子及其胚轴的干燥耐受性和冷冻耐受性进行了研究。种子通过硅胶、在层流通风柜中或放置在实验室工作台上进行干燥处理。无论采用何种干燥方法，无种皮种子在冷冻保存前后的存活率更高。冷冻完整种子时，在实验室工作台上干燥至含水量7.3%（鲜重基础）后，获得最高存活率（41.3%）。在层流通风柜中或实验室工作台上干燥后，无种皮冷冻保存种子的存活率分别可达85%，对应的含水量分别为7.1%和4.5%。用硅胶干燥后，种子含水量为3.3%时，存活率最高达到60.0%。无论预培养基中的蔗糖浓度和干燥时间如何，对照胚轴的存活率都很高（80 - 100%）。冷冻保存后，未进行预培养和干燥的胚轴没有存活率。冷冻保存后，未干燥胚轴的存活率随着预培养基中蔗糖浓度的增加而逐渐增加，从0.1 M蔗糖时的7.5%增加到0.7 M蔗糖时的77.5%。无论预培养处理和干燥时间如何，干燥并冷冻保存的胚的存活率始终很高，范围在55.8%至92.5%之间。
