Tyson John J, Csikasz-Nagy Attila, Novak Bela
Department of Biology, Virginia Polytechnic Institute & State University, Blacksburg 24061, USA.
Bioessays. 2002 Dec;24(12):1095-109. doi: 10.1002/bies.10191.
Major events of the cell cycle--DNA synthesis, mitosis and cell division-are regulated by a complex network of protein interactions that control the activities of cyclin-dependent kinases. The network can be modeled by a set of nonlinear differential equations and its behavior predicted by numerical simulation. Computer simulations are necessary for detailed quantitative comparisons between theory and experiment, but they give little insight into the qualitative dynamics of the control system and how molecular interactions determine the fundamental physiological properties of cell replication. To that end, bifurcation diagrams are a useful analytical tool, providing new views of the dynamical organization of the cell cycle, the role of checkpoints in assuring the integrity of the genome, and the abnormal regulation of cell cycle events in mutants. These claims are demonstrated by an analysis of cell cycle regulation in fission yeast.
细胞周期的主要事件——DNA合成、有丝分裂和细胞分裂——受一个复杂的蛋白质相互作用网络调控，该网络控制着细胞周期蛋白依赖性激酶的活性。这个网络可用一组非线性微分方程建模，并通过数值模拟预测其行为。计算机模拟对于理论与实验之间的详细定量比较是必要的，但它们对于控制系统的定性动力学以及分子相互作用如何决定细胞复制的基本生理特性几乎没有提供深入见解。为此，分岔图是一种有用的分析工具，它能提供关于细胞周期动态组织、检查点在确保基因组完整性中的作用以及突变体中细胞周期事件异常调控的新观点。对裂殖酵母细胞周期调控的分析证明了这些观点。
