绿色荧光蛋白在转基因植物中的应用。

The utility of green fluorescent protein in transgenic plants.

作者信息

Stewart C N

机构信息

Department of Biology, University of North Carolina-Greensboro, NC 27402-6174, USA.

出版信息

Plant Cell Rep. 2001 Jul;20(5):376-82. doi: 10.1007/s002990100346.

DOI:10.1007/s002990100346

PMID:12448420

Abstract

The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has proven to be a powerful tool in plant genetic transformation studies. This paper reviews the history and the progression of the expression of GFP variants in transgenic plants. The distinguishing features of the most useful GFPs, such as those including the S65T chromophore mutation and those with dual excitation peaks, are discussed. The review also focuses on the utility of GFP as a visual selectable marker in aiding the plant transformation process; GFP has been more important in monocot transformation compared with dicot transformation. Finally, the potential utility of new fluorescent proteins is speculated upon.

摘要

来自维多利亚多管水母的绿色荧光蛋白（GFP）已被证明是植物遗传转化研究中的一种强大工具。本文综述了绿色荧光蛋白变体在转基因植物中表达的历史和进展。讨论了最有用的绿色荧光蛋白的显著特征，例如那些包含S65T发色团突变的绿色荧光蛋白以及具有双激发峰的绿色荧光蛋白。该综述还着重介绍了绿色荧光蛋白作为视觉选择标记在辅助植物转化过程中的作用；与双子叶植物转化相比，绿色荧光蛋白在单子叶植物转化中更为重要。最后，推测了新型荧光蛋白的潜在用途。