Reddy Srinivasa T, Grijalva Victor, Ng Carey, Hassan Khaled, Hama Susan, Mottahedeh Rachel, Wadleigh David J, Navab Mohamad, Fogelman Alan M
Atherosclerosis Research Unit, Department of Medicine, Department of Molecular and Medical Pharmacology, University of California Los Angeles, 650 Charles E. Young Drive South, A8-131 CHS, Los Angeles, CA 90095, USA.
Vascul Pharmacol. 2002 Apr;38(4):211-8. doi: 10.1016/s1537-1891(02)00171-4.
Oxidized-L-alpha-1-Palmitoyl-2-Arachidonoyl-sn-glycero-3-Phosphorylcholine (Ox-PAPC), a component of mildly oxidized/minimally modified low-density lipoprotein (MM-LDL), accounts for many of the biological activities of MM-LDL. Having hypothesized that Ox-PAPC initiates gene expression changes in endothelial cells that result in enhanced endothelial/monocyte interactions and the subsequent development of atherosclerotic lesions, we used the suppression subtractive hybridization (SSH) procedure to compare mRNA isolated from PAPC-treated human aortic endothelial cells (HAEC) with mRNA isolated from Ox-PAPC-treated cells. Genes induced by Ox-PAPC but not by PAPC in HAEC included genes involved in signal transduction, extracellular matrix, growth factors, chemokines and several genes with unknown functions. The observed pattern of gene induction suggests that Ox-PAPC may play multiple roles in angiogenesis, atherosclerosis, and inflammation and wound healing.
氧化型L-α-1-棕榈酰-2-花生四烯酰-sn-甘油-3-磷酸胆碱（Ox-PAPC）是轻度氧化/最低限度修饰的低密度脂蛋白（MM-LDL）的一种成分，它介导了MM-LDL的许多生物学活性。我们推测Ox-PAPC会引发内皮细胞中的基因表达变化，进而导致内皮细胞/单核细胞相互作用增强以及随后动脉粥样硬化病变的发展。于是，我们采用抑制性消减杂交（SSH）技术，比较了从PAPC处理的人主动脉内皮细胞（HAEC）中分离的mRNA与从Ox-PAPC处理的细胞中分离的mRNA。在HAEC中，由Ox-PAPC而非PAPC诱导的基因包括参与信号转导、细胞外基质、生长因子、趋化因子的基因以及几个功能未知的基因。观察到的基因诱导模式表明，Ox-PAPC可能在血管生成、动脉粥样硬化、炎症和伤口愈合中发挥多种作用。
