Benites N R, Guerra J L, Melville P A, da Costa E O
Research Nucleus on Mammary Gland and Milk Production, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Brazil.
J Vet Med B Infect Dis Vet Public Health. 2002 Oct;49(8):366-70. doi: 10.1046/j.1439-0450.2002.00566.x.
Infectious bovine mastitis is responsible for serious economic losses in dairy cattle breeding. Information about the damages caused by micro-organisms is of great interest and importance. The purpose of the present study was to report on the microbiological and histopathological aspects of the mammary parenchymas of slaughtered dairy cows. A total of 184 mammary glands were examined and samples of mammary parenchyma were collected for microbiological and histopathological examinations. Micro-organisms were isolated from 69.6% samples; 23 (12.5%) of the 184 samples did not show histological changes; inflammatory response was observed in 56 (30.4%) samples; inflammatory response and repair were present in 82 (44.6%) samples; repair process was verified in 23 (12.5%) of the mammary glands. Coagulase-negative Staphylococci were observed in 53.8% of the samples followed by coagulase-positive Staphylococci (7.6%), Prototheca sp. (2.2%) and Streptococcus dysgalactiae (1.6%). Coagulase-negative Staphylococci and coagulase-positive Staphylococci were associated mainly to chronic inflammatory response and chronic inflammatory response and repair. Samples from which no micro-organisms were isolated (n = 56) had no histological changes in 82.6% of the cases. These results were higher (P < 0.05) when compared to the samples with micro-organisms and without histological changes (17.4%).
传染性牛乳腺炎给奶牛养殖带来了严重的经济损失。关于微生物所造成损害的信息极具研究价值和重要性。本研究的目的是报告屠宰奶牛乳腺实质的微生物学和组织病理学方面的情况。共检查了184个乳腺，并采集了乳腺实质样本进行微生物学和组织病理学检查。从69.6%的样本中分离出微生物；184个样本中有23个（12.5%）未显示组织学变化；56个（30.4%）样本观察到炎症反应；82个（44.6%）样本存在炎症反应和修复；23个（12.5%）乳腺证实有修复过程。53.8%的样本中观察到凝固酶阴性葡萄球菌，其次是凝固酶阳性葡萄球菌（7.6%）、原壁菌属（2.2%）和停乳链球菌（1.6%）。凝固酶阴性葡萄球菌和凝固酶阳性葡萄球菌主要与慢性炎症反应以及慢性炎症反应和修复有关。未分离出微生物的样本（n = 56）中，82.6%的病例无组织学变化。与有微生物但无组织学变化的样本（17.4%）相比，这些结果更高（P < 0.05）。
