Efimov B A, Kafarskaia L I, Korshunov V M
State Medical University, Institute of Medico-Social Rehabilitology, Moscow, Russia.
Zh Mikrobiol Epidemiol Immunobiol. 2002 Jul-Aug(4):72-8.
Disturbances in normal intestinal and vaginal microflora in women have recently become quite frequent. This accounts for the need of bacteriological laboratories for introduction of reliable methods for the diagnosis of such disturbances. Correct methodological approaches to objective evaluation of the state of intestinal and vaginal microflora are described. The methods used in the study of anaerobic microflora (lacto- and bifidobacteria, eubacteria, peptostreptococci, clostridia, bacteriods, fusobacteria) and facultative anaerobic microorganisms (enterobacteria, staphylococci, streptococci, Gardnerella, fungi of the genus Candida) have been analyzed. All stages of the study are described in consecutive order: the transportation of the material under study, its treatment in a laboratory, the spectrum of selective nutrient media for the isolation of microorganisms, methods of their identification.
近年来,女性正常肠道和阴道微生物群紊乱的情况变得相当频繁。这说明了细菌学实验室需要引入可靠的方法来诊断此类紊乱。文中描述了客观评估肠道和阴道微生物群状态的正确方法学途径。分析了用于研究厌氧微生物群(乳酸杆菌和双歧杆菌、真细菌、消化链球菌、梭菌、拟杆菌、梭杆菌)和兼性厌氧微生物(肠杆菌、葡萄球菌、链球菌、加德纳菌、念珠菌属真菌)的方法。研究的所有阶段按顺序进行了描述:所研究材料的运输、在实验室中的处理、用于分离微生物的选择性营养培养基的种类、微生物鉴定方法。