Sugiyama Kimio, Shimada Yasuhiko, Iwai Kasumi, Morita Tatsuya
Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529, Japan.
Biosci Biotechnol Biochem. 2002 Oct;66(10):2232-5. doi: 10.1271/bbb.66.2232.
Effects of dietary protein type on lipopolysaccharide (LPS)-induced hepatitis, as assessed by plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, were investigated in D-galactosamine (GalN)-sensitized rats. The plasma ALT and AST activities in rats fed on 25% soybean protein isolate (SPI) diet were significantly suppressed to about 1/4 and 1/5 of the values in rats fed on 25% casein diet, respectively, 8 h after the injection of LPS + GalN. Although hepatic ALT and AST activities of normal rats were also lower in the SPI group than in the casein group, this could not explain the differences in plasma enzyme activities between the two groups. The hepatic glutathione concentration of normal rats was lower in the SPI group than in the casein group, but it was reversed in rats injected with drugs. The results suggest that SPI can protect animals from LPS + GalN-induced hepatitis, and that the hepatic glutathione level may participate in the effects of SPI.
通过血浆丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）活性评估膳食蛋白质类型对脂多糖（LPS）诱导的肝炎的影响，在D - 半乳糖胺（GalN）致敏的大鼠中进行了研究。注射LPS + GalN 8小时后，喂食25%大豆分离蛋白（SPI）饮食的大鼠血浆ALT和AST活性分别显著抑制至喂食25%酪蛋白饮食大鼠的约1/4和1/5。尽管正常大鼠的肝脏ALT和AST活性在SPI组中也低于酪蛋白组，但这无法解释两组之间血浆酶活性的差异。正常大鼠肝脏谷胱甘肽浓度在SPI组中低于酪蛋白组，但在注射药物的大鼠中则相反。结果表明，SPI可以保护动物免受LPS + GalN诱导的肝炎，并且肝脏谷胱甘肽水平可能参与了SPI的作用。
