含锗螺旋藻对（D）-半乳糖胺和脂多糖诱导的肝炎大鼠的肝保护作用。

Hepatoprotective effect of germanium-containing Spirulina in rats with (D)-galactosamine- and lipopolysaccharide-induced hepatitis.

机构信息

Department of Bioresource Engineering, Faculty of Agriculture, Yamagata University, Yamagata 997-8555, Japan.

Ryusendo Company Limited, Tokyo 171-0021, Japan.

出版信息

Br J Nutr. 2014 Jan 14;111(1):135-40. doi: 10.1017/S0007114513001943. Epub 2013 Jun 17.

DOI:10.1017/S0007114513001943

PMID:23768655

Abstract

In the present study, the protective effects of dietary Spirulina (SP) and germanium-containing Spirulina (GeSP) were compared in rats with liver injury induced by an intraperitoneal injection of d-galactosamine and lipopolysaccharide (GalN/LPS). Wistar rats were fed one of the following diets: the basal diet (GalN/LPS-CON group; n 6), the basal diet supplemented with 5 % SP or GeSP (GalN/LPS-SP and GalN/LPS-GeSP group, respectively; n 7 each). After administering these diets for 7 d, each rat was intraperitoneally injected with GalN/LPS. Increases in plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were suppressed in the GalN/LPS-GeSP group (GalN/LPS-CON v. GalN/LPS-GeSP: ALT 1052 (sem 187) v. 509 (sem 88) IU/l and AST 2183 (sem 368) v. 1170 (sem 196) IU/l) following the injection of GalN/LPS. Plasma levels of interferon-γ (IFN-γ) and TNF-α in GeSP-fed rats were significantly lower when compared with those in the GalN/LPS-CON group (GalN/LPS-CON v. GalN/LPS-GeSP: IFN-γ 142·8 (sem 17·5) v. 66·8 (sem 9·7) pg/ml and TNF-α 72·3 (sem 15·4) v. 31·2 (sem 6·8) pg/ml). However, the decrease in these levels observed in the GalN/LPS-SP group was not as prominent as those observed in the GalN/LPS-GeSP group. Furthermore, the increase in liver catalase (CAT) and glutathione peroxidase (GPx) activities, as well as the level of oxidised glutathione (GSSG), was more suppressed in GeSP-fed rats (GalN/LPS-CON v. GalN/LPS-GeSP: CAT 457 (sem 47) v. 262 (sem 54) U/mg liver protein; GPx 1·30 (sem 0·11) v. 0·53 (sem 0·09) U/mg liver protein; GSSG 2·18 (sem 0·33) v. 1·31 (sem 0·24) mmol/kg liver) after the injection of GalN/LPS. These changes were more pronounced in the GalN/LPS-GeSP group than in the GalN/LPS-SP group. These results suggest that GeSP could afford a significant protective effect in the alleviation of GalN/LPS-induced hepatic damage. In addition, the results indicate that GeSP is more effective than SP.

摘要

在本研究中，比较了膳食螺旋藻（SP）和含锗螺旋藻（GeSP）对腹腔注射半乳糖胺和脂多糖（GalN/LPS）诱导的肝损伤大鼠的保护作用。Wistar 大鼠分别喂食以下饮食之一：基础饮食（GalN/LPS-CON 组；n 6）、基础饮食中添加 5% SP 或 GeSP（GalN/LPS-SP 和 GalN/LPS-GeSP 组，分别为 n 7）。喂食这些饮食 7 天后，每只大鼠均腹腔注射 GalN/LPS。GalN/LPS-GeSP 组大鼠血浆丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）活性升高（GalN/LPS-CON v. GalN/LPS-GeSP：ALT 1052（sem 187）v. 509（sem 88）IU/l 和 AST 2183（sem 368）v. 1170（sem 196）IU/l）GalN/LPS 注射后。与 GalN/LPS-CON 组相比，GeSP 喂养大鼠的血浆干扰素-γ（IFN-γ）和 TNF-α 水平明显降低（GalN/LPS-CON v. GalN/LPS-GeSP：IFN-γ 142.8（sem 17.5）v. 66.8（sem 9.7）pg/ml 和 TNF-α 72.3（sem 15.4）v. 31.2（sem 6.8）pg/ml）。然而，GalN/LPS-SP 组观察到的这些水平下降并不如 GalN/LPS-GeSP 组那么明显。此外，GalN/LPS-GeSP 喂养大鼠的肝过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）活性升高以及氧化型谷胱甘肽（GSSG）水平升高更为显著（GalN/LPS-CON v. GalN/LPS-GeSP：CAT 457（sem 47）v. 262（sem 54）U/mg 肝蛋白；GPx 1.30（sem 0.11）v. 0.53（sem 0.09）U/mg 肝蛋白；GSSG 2.18（sem 0.33）v. 1.31（sem 0.24）mmol/kg 肝）GalN/LPS 注射后。GalN/LPS-GeSP 组的这些变化比 GalN/LPS-SP 组更为明显。这些结果表明，GeSP 可显著减轻 GalN/LPS 诱导的肝损伤。此外，结果表明 GeSP 比 SP 更有效。