de Champlain J, Farley L, Cousineau D, van Ameringen M R
Circ Res. 1976 Feb;38(2):109-14. doi: 10.1161/01.res.38.2.109.
The radiometric enzymatic technique of Coyle and Henry (J. Neurochem. 21: 61-67, 1973) was adapted to the measurement of serum catecholamines. This technique requires less time than other enzymatic techniques and is sensitive to quantities as small as 25 pg. In normotensive subjects lying supine for 20 minutes serum catecholamine levels averaged 0.218 ng/ml, with no obvious sex or age difference. Under these standardized conditions, the circulating catecholamine levels for a given individual are highly reproducible on different days over a period of several months. In 22 patients with essential hypertension, circulating levels were significantly higher, with an average of 0.370 ng/ml. More than 50% of the hypertensive patients had values greater than the highest value measured in normotensives. Systolic blood pressure and heart rate were significantly higher in the hypertensive group with elevated levels of circulating catecholamines than in the hypertensive group with normal levels. In one model of experimental hypertension, produced in the rat by administration of deoxycorticosterone acetate (DOCA) and saline for 4-8 weeks, serum catecholamines were significantly elevated. These findings suggest that the sympathetic system may play an important role in maintaining an elevated blood pressure in experimental hypertension and in a significant proportion of patients with essential hypertension.
科伊尔和亨利的放射性酶技术（《神经化学杂志》21: 61 - 67, 1973）被用于血清儿茶酚胺的测量。该技术所需时间比其他酶技术少，对低至25皮克的量也很敏感。在仰卧20分钟的血压正常受试者中，血清儿茶酚胺水平平均为0.218纳克/毫升，无明显性别或年龄差异。在这些标准化条件下，给定个体的循环儿茶酚胺水平在几个月的不同日子里具有高度可重复性。在22例原发性高血压患者中，循环水平显著更高，平均为0.370纳克/毫升。超过50%的高血压患者的值高于血压正常者测量的最高值。循环儿茶酚胺水平升高的高血压组的收缩压和心率显著高于循环水平正常的高血压组。在通过给予醋酸脱氧皮质酮（DOCA）和生理盐水4 - 8周在大鼠中产生的一种实验性高血压模型中，血清儿茶酚胺显著升高。这些发现表明，交感神经系统可能在实验性高血压以及相当一部分原发性高血压患者维持血压升高方面发挥重要作用。
