Zhu Zhimeng, Neirinck Len
Pharmascience, 6111 Royalmount Ave., Suite 100, Quebec, H4P 2T4, Montreal, Canada.
J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Jan 5;783(1):133-40. doi: 10.1016/s1570-0232(02)00526-3.
A new method, using high-performance liquid chromatography/ion electrospray (negative ion) mass spectrometry, has been developed for the determination of a hydrophilic liver-specific inhibitor of the enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase, pravastatin in human plasma. In this method, plasma samples were prepared by a solid-phase extraction on C(18) Bond Elut cartridge. Chromatography was carried out with a Zorbax C(8) column. Simple isocratic chromatography conditions were used. The method has been validated in a linear range of 0.25-300 ng/ml with a coefficient of variation of 0.6-3.4%. The overall recovery was 90.5% for pravastatin and 90.8% for the internal standard beta-hydroxy-lovastatin. The method is simple and reliable with a total run time of less than 2 min.
已开发出一种采用高效液相色谱/离子电喷雾（负离子）质谱法测定人血浆中3-羟基-3-甲基戊二酰辅酶A还原酶的亲水性肝脏特异性抑制剂普伐他汀的新方法。在此方法中，血浆样品通过在C(18) Bond Elut柱上进行固相萃取来制备。色谱分离采用Zorbax C(8)柱进行。使用简单的等度色谱条件。该方法在0.25 - 300 ng/ml的线性范围内得到验证，变异系数为0.6 - 3.4%。普伐他汀的总回收率为90.5%，内标β-羟基洛伐他汀的总回收率为90.8%。该方法简单可靠，总运行时间不到2分钟。
