Keane P M, Walker W H, Gauldie J, Abraham G E
Clin Chem. 1976 Jan;22(1):70-3.
In a number of radioimmunoassays and radiotransin assays, effective equilibrium constants have been measured at different temperatures in order to define the relative contribution of changes of entropy and enthalpy to the change in free binding energy. In systems with a large enthalpy component, the lowest possible incubation temperature maximizes sensitivity, and control of temperature throughout the assay is important. Conversely, when enthalpy change is small, a high temperature allows rapid attainment of equilibrium without loss of sensitivity. At a theoretical level, the thermodynamic characteristics of binding may allow some insight into the nature of the binding process.
在一些放射免疫分析和放射转铁蛋白分析中，已在不同温度下测量了有效平衡常数，以便确定熵变和焓变对自由结合能变化的相对贡献。在具有较大焓成分的系统中，尽可能低的孵育温度可使灵敏度最大化，并且在整个分析过程中控制温度很重要。相反，当焓变较小时，高温可使快速达到平衡而不损失灵敏度。从理论层面来看，结合的热力学特性可能有助于深入了解结合过程的本质。
