用于定量与博氏块菌菌丝体相关的噬纤维菌-屈挠杆菌-拟杆菌门细菌的竞争性PCR。
Competitive PCR for quantitation of a Cytophaga-Flexibacter-Bacteroides phylum bacterium associated with the Tuber borchii Vittad. mycelium.
作者信息
Barbieri Elena, Riccioni Giulia, Pisano Anna, Sisti Davide, Zeppa Sabrina, Agostini Deborah, Stocchi Vilberto
机构信息
Giorgio Fornaini Institute of Biochemistry, University of Urbino, Pesaro-Urbino, Italy.
出版信息
Appl Environ Microbiol. 2002 Dec;68(12):6421-4. doi: 10.1128/AEM.68.12.6421-6424.2002.
Abstract
An uncultured bacterium associated with the ectomycorrhizal fungus Tuber borchii Vittad. was identified as a novel member of the Cytophaga-Flexibacter-Bacteroides group. Utilizing a quantitative PCR targeting the 16S rRNA gene, we relatively quantified this bacterium in the host. The estimated number of bacteria was found to be approximately 10(6) cells per 30-day-old T. borchii mycelium culture. This represents the first molecular attempt to enumerate an uncultured bacterium associated with a mycorrhizal fungus.
摘要
一种与外生菌根真菌白块菌（Tuber borchii Vittad.）相关的未培养细菌被鉴定为噬纤维菌-屈挠杆菌-拟杆菌菌群的一个新成员。利用靶向16S rRNA基因的定量PCR技术，我们对宿主中的这种细菌进行了相对定量分析。结果发现，每30日龄的白块菌菌丝体培养物中细菌的估计数量约为10⁶个细胞。这是首次尝试从分子层面计数与菌根真菌相关的未培养细菌。
相似文献
1
Competitive PCR for quantitation of a Cytophaga-Flexibacter-Bacteroides phylum bacterium associated with the Tuber borchii Vittad. mycelium.用于定量与博氏块菌菌丝体相关的噬纤维菌-屈挠杆菌-拟杆菌门细菌的竞争性PCR。
Appl Environ Microbiol. 2002 Dec;68(12):6421-4. doi: 10.1128/AEM.68.12.6421-6424.2002.
2
Phylogenetic characterization and in situ detection of a Cytophaga-Flexibacter-Bacteroides phylogroup bacterium in Tuber borchii vittad. Ectomycorrhizal mycelium.块菌外生菌根菌丝体中噬纤维菌-屈挠杆菌-拟杆菌菌群细菌的系统发育特征及原位检测
Appl Environ Microbiol. 2000 Nov;66(11):5035-42. doi: 10.1128/AEM.66.11.5035-5042.2000.
3
Reichenbachia agariperforans gen. nov., sp. nov., a novel marine bacterium in the phylum Cytophaga-Flavobacterium-Bacteroides.穿琼脂赖氏菌，新属，新种，噬纤维菌-黄杆菌-拟杆菌门中的一种新型海洋细菌。
Int J Syst Evol Microbiol. 2003 Jan;53(Pt 1):81-85. doi: 10.1099/ijs.0.02128-0.
4
Application of a suite of 16S rRNA-specific oligonucleotide probes designed to investigate bacteria of the phylum cytophaga-flavobacter-bacteroides in the natural environment.一套旨在研究自然环境中噬纤维菌-黄杆菌-拟杆菌门细菌的16S rRNA特异性寡核苷酸探针的应用。
Microbiology (Reading). 1996 May;142 ( Pt 5):1097-1106. doi: 10.1099/13500872-142-5-1097.
5
Phylogenetic position of Chitinophaga pinensis in the Flexibacter-Bacteroides-Cytophaga phylum.食松几丁质噬菌在柔杆菌-拟杆菌-噬纤维菌门中的系统发育位置。
Int J Syst Bacteriol. 1999 Apr;49 Pt 2:479-81. doi: 10.1099/00207713-49-2-479.
6
16S rRNA-targeted oligonucleotide probes for the in situ detection of members of the phylum Cytophaga-Flavobacterium-Bacteroides.用于原位检测噬纤维菌-黄杆菌-拟杆菌门成员的16S rRNA靶向寡核苷酸探针。
Syst Appl Microbiol. 2000 Apr;23(1):107-14. doi: 10.1016/S0723-2020(00)80051-X.
7
New degenerate Cytophaga-Flexibacter-Bacteroides-specific 16S ribosomal DNA-targeted oligonucleotide probes reveal high bacterial diversity in River Taff epilithon.新型简并噬纤维菌-屈挠杆菌-拟杆菌特异性16S核糖体DNA靶向寡核苷酸探针揭示了塔夫河中表生生物膜具有高度的细菌多样性。
Appl Environ Microbiol. 2002 Jan;68(1):201-10. doi: 10.1128/AEM.68.1.201-210.2002.
8
Extremely halophilic bacteria in crystallizer ponds from solar salterns.来自太阳能盐场结晶池的极端嗜盐细菌。
Appl Environ Microbiol. 2000 Jul;66(7):3052-7. doi: 10.1128/AEM.66.7.3052-3057.2000.
9
Chryseobacterium defluvii sp. nov., isolated from wastewater.从废水中分离出的新种河流金色杆菌。
Int J Syst Evol Microbiol. 2003 Jan;53(Pt 1):93-97. doi: 10.1099/ijs.0.02073-0.
10
The sequence of the single 16S rRNA gene of the thermophilic eubacterium Rhodothermus marinus reveals a distant relationship to the group containing Flexibacter, Bacteroides, and Cytophaga species.
J Bacteriol. 1994 Oct;176(20):6165-9. doi: 10.1128/jb.176.20.6165-6169.1994.
引用本文的文献
1
Bacterial Endosymbionts: Master Modulators of Fungal Phenotypes.细菌内共生体：真菌表型的主要调控因子。
Microbiol Spectr. 2017 Sep;5(5). doi: 10.1128/microbiolspec.FUNK-0056-2016.
2
Bacteria of the genus Rhodopseudomonas (Bradyrhizobiaceae): obligate symbionts in mycelial cultures of the black truffles Tuber melanosporum and Tuber brumale.
Springerplus. 2016 Jul 15;5(1):1085. doi: 10.1186/s40064-016-2756-6. eCollection 2016.
3
Intraspecific genotypic variability determines concentrations of key truffle volatiles.种内基因型变异性决定了关键松露挥发物的浓度。
New Phytol. 2012 May;194(3):823-835. doi: 10.1111/j.1469-8137.2012.04077.x. Epub 2012 Mar 6.
本文引用的文献
1
Isolation and characterization of differentially expressed genes in the mycelium and fruit body of Tuber borchii.波氏块菌菌丝体和子实体中差异表达基因的分离与鉴定
Appl Environ Microbiol. 2002 Sep;68(9):4574-82. doi: 10.1128/AEM.68.9.4574-4582.2002.
2
Identification of differentially expressed cDNA clones in Tilia platyphyllos-Tuber borchii ectomycorrhizae using a differential screening approach.利用差异筛选方法鉴定阔叶椴-博氏块菌外生菌根中差异表达的cDNA克隆。
Mol Genet Genomics. 2002 Jan;266(5):858-64. doi: 10.1007/s00438-001-0607-8. Epub 2001 Nov 16.
3
A partial phylogenetic analysis of the "flavobacter-bacteroides" phylum: basis for taxonomic restructuring.“黄杆菌-拟杆菌”门的部分系统发育分析：分类重组的基础
Syst Appl Microbiol. 1992 Dec;15(4):513-21. doi: 10.1016/S0723-2020(11)80110-4.
4
rrndb: the Ribosomal RNA Operon Copy Number Database.rrndb：核糖体RNA操纵子拷贝数数据库。
Nucleic Acids Res. 2001 Jan 1;29(1):181-4. doi: 10.1093/nar/29.1.181.
5
Phylogenetic characterization and in situ detection of a Cytophaga-Flexibacter-Bacteroides phylogroup bacterium in Tuber borchii vittad. Ectomycorrhizal mycelium.块菌外生菌根菌丝体中噬纤维菌-屈挠杆菌-拟杆菌菌群细菌的系统发育特征及原位检测
Appl Environ Microbiol. 2000 Nov;66(11):5035-42. doi: 10.1128/AEM.66.11.5035-5042.2000.
6
Basic principles of quantitative PCR.
Mol Biotechnol. 2000 Jun;15(2):115-22. doi: 10.1385/MB:15:2:115.
7
Quantitative selective PCR of 16S ribosomal DNA correlates well with selective agar plating in describing population dynamics of indigenous Pseudomonas spp. in soil hot spots.16S核糖体DNA的定量选择性聚合酶链反应在描述土壤热点中本地假单胞菌属种群动态方面与选择性琼脂平板培养法相关性良好。
Appl Environ Microbiol. 1999 Apr;65(4):1786-8. doi: 10.1128/AEM.65.4.1786-1788.1999.
8
Classification of heparinolytic bacteria into a new genus, Pedobacter, comprising four species: Pedobacter heparinus comb. nov., Pedobacter piscium comb. nov., Pedobacter africanus sp. nov. and Pedobacter saltans sp. nov. proposal of the family Sphingobacteriaceae fam. nov.将解肝素细菌分类为一个新属，即食酸菌属，包括四个物种：食酸解肝素菌组合新种、食酸解鱼素菌组合新种、非洲食酸菌新种和跳跃食酸菌新种。提出鞘脂杆菌科新科。
Int J Syst Bacteriol. 1998 Jan;48 Pt 1:165-77. doi: 10.1099/00207713-48-1-165.
9
Detection of Clostridium proteoclasticum and closely related strains in the rumen by competitive PCR.通过竞争性聚合酶链反应检测瘤胃中的解蛋白梭菌及密切相关菌株。
Appl Environ Microbiol. 1998 Mar;64(3):907-13. doi: 10.1128/AEM.64.3.907-913.1998.
10
Technical aspects of quantitative competitive PCR.定量竞争性聚合酶链反应的技术方面
Biotechniques. 1996 Aug;21(2):268-72, 274-9. doi: 10.2144/96212rv01.
Zotero 插件