Kämpfer Peter, Dreyer Ute, Neef Alexander, Dott Wolfgang, Busse Hans-Jürgen
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26-32, D-35390 Giessen, Germany.
Fachgebiet Hygiene und Umweltmikrobiologie, Technische Universität Berlin, D-13353 Berlin, Germany.
Int J Syst Evol Microbiol. 2003 Jan;53(Pt 1):93-97. doi: 10.1099/ijs.0.02073-0.
A Gram-negative, rod-shaped, non-spore-forming, yellow-pigmented bacterium (strain B2T) isolated from wastewater of a sequence batch reactor showing enhanced phosphorus removal was investigated to determine its taxonomic status. Complete 16S rRNA gene sequence analysis indicated that the organism should be placed in the genus Chryseobacterium. The strain contained a polyamine pattern with sym-homospermidine as the major compound, menaquinone MK-6 as the predominant menaquinone and ai-C15:0, i-C15:0 and C16:1 as the major fatty acids. Phosphatidylethanolamine and several unidentified lipids were detected in the polar lipid profile. Phylogenetically, strain B2T was most closely related to Chryseobacterium indoltheticum and Chryseobacterium gleum (96.2 and 95-9% 16S rRNA gene sequence similarity, respectively). The phylogenetic distance from any validly described species within the genus Chryseobacterium, as indicated from 16S rRNA gene sequence similarities, and its phenotypic properties demonstrate that strain B2T represents a novel species, for which the name Chryseobacterium defluvii sp. nov. is proposed; the type strain is B2T (=DSM 14219T =CIP 107207T).
从一个显示出强化除磷的序批式反应器废水中分离出一株革兰氏阴性、杆状、不产芽孢、产黄色色素的细菌（菌株B2T），对其进行研究以确定其分类地位。完整的16S rRNA基因序列分析表明该微生物应归入金黄杆菌属。该菌株的多胺模式以对称高亚精胺为主要化合物，以甲基萘醌MK-6为主要甲基萘醌，以ai-C15:0、i-C15:0和C16:1为主要脂肪酸。在极性脂质谱中检测到磷脂酰乙醇胺和几种未鉴定的脂质。从系统发育来看，菌株B2T与吲哚金黄杆菌和黏金黄杆菌关系最为密切（16S rRNA基因序列相似性分别为96.2%和95.9%）。16S rRNA基因序列相似性表明，该菌株与金黄杆菌属内任何已有效描述的物种之间的系统发育距离及其表型特征表明，菌株B2T代表一个新物种，为此提出金黄杆菌属新种名称为污水金黄杆菌（Chryseobacterium defluvii sp. nov.）；模式菌株为B2T（=DSM 14219T =CIP 107207T）。
