Egorov N S, Kochetov G A, Khaĭdarova N V
Mikrobiologiia. 1976 May-Jun;45:455-9.
A fibrinolytic enzyme was isolated from the cultural broth of Actinomyces thermovulgaris T-54 by precipitation with ammonium sulphate to 0.8 saturation and chromatography on GE-cellulose and CM-cellulose. The enzyme is homogeneous as was confirmed by disk electrophoresis in polyacrylamide gel. Some properties of the enzyme were studied. A modification of the method is suggested to assay the fibrinolytic activity.