González Salvador, Tannous Zeina
Wellman Laboratories of Photomedicine, Department of Dermatology, Massachusetts General Hospital, Harvard Medical School, Boston 02114, USA.
J Am Acad Dermatol. 2002 Dec;47(6):869-74. doi: 10.1067/mjd.2002.124690.
Real-time, near-infrared confocal laser scanning microscopy may provide a way to diagnose basal cell carcinoma in vivo and might potentially eliminate the need for invasive diagnostic biopsies in the future.
The purpose of this study is to define the in vivo histologic features of basal cell carcinoma by using a high-resolution imaging technique.
Five fair-skinned white patients with 8 basal cell carcinoma lesions were recruited for this study. Near-infrared reflectance confocal microscopy imaging was used to characterize the histologic features of these lesions in vivo. Subsequently, the confocal histologic features were correlated with the corresponding routine hematoxylin-and-eosin-stained sections obtained from invasive biopsies.
A uniform population of basal cell carcinoma cells with characteristic elongated nuclei oriented along the same axis was always present. Abundant blood vessels demonstrating prominent tortuosity were seen, as well as prominent, predominantly mononuclear inflammatory infiltrate admixed or in close apposition with basal cell carcinoma cells. Trafficking of leukocytes was visualized in real time.
Our results demonstrate that near-infrared confocal microscopy may facilitate diagnosis of basal cell carcinoma with the use of in vivo high-resolution confocal features. Accuracy studies to evaluate these in vivo histologic criteria are warranted.
实时近红外共聚焦激光扫描显微镜检查可能为基底细胞癌的体内诊断提供一种方法，并且未来可能无需进行侵入性诊断活检。
本研究的目的是使用高分辨率成像技术确定基底细胞癌的体内组织学特征。
招募了5名患有8个基底细胞癌病变的白皮肤白人患者参与本研究。使用近红外反射共聚焦显微镜成像来表征这些病变的体内组织学特征。随后，将共聚焦组织学特征与从侵入性活检获得的相应常规苏木精-伊红染色切片进行关联。
始终存在一群均匀的基底细胞癌细胞，其特征性的细长核沿同一轴排列。可见大量显示明显迂曲的血管，以及与基底细胞癌细胞混合或紧密相邻的突出的、主要为单核的炎性浸润。实时观察到白细胞的游走。
我们的结果表明，近红外共聚焦显微镜检查可利用体内高分辨率共聚焦特征促进基底细胞癌的诊断。有必要进行准确性研究以评估这些体内组织学标准。
