Mechanisms involved in the vasodilator effect of curine in rat resistance arteries.

The vasodilator effect of curine was investigated in the rat small mesenteric arteries. In either endothelium-intact or endothelium-denuded mesenteric arteries, curine induced a concentration-dependent relaxation in rings pre-contracted with noradrenline (10 microM; IC 50 = 4.8 +/- 1.3 microM and 4.8 +/- 1.5 microM, respectively) and KCl (80 mM; IC 50 = 6.0 +/- 1.3 microM and 13.0 +/- 5.6 microM, respectively). Curine also inhibited (IC 50 = 4.6 +/- 0.9 microM) the concentration-response curves induced by noradrenaline. Contractions dependent on calcium-influx elicited by KCl (80 mM) and noradrenaline (10 microM) were inhibited by curine (10 microM). Finally, contractions induced by noradrenaline (10 microM), in calcium-free medium, were strongly inhibited by curine (10 microM). The above results suggest that the inhibition of influx of calcium ions through voltage-operated calcium channels and non-selective channels, and mobilization of intracellular calcium stores sensitive to noradrenaline are involved in the vasodilator effect of curine.