Ding Xue-qiang, Wang An-xun, Kong Qing-yu, Chen Hui-zhi, Chen Yu
Department of Stomatology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, P. R. China.
Ai Zheng. 2002 Apr;21(4):388-91.
BACKGROUND & OBJECTIVE: Studies have demonstrated that hydroxycamptothecin (HCPT) had therapeutic effect on many malignant tumors, but few studies on oral squamous cell carcinoma were reported. This study was designed to investigate the anticancer effect and mechanism of hydroxycampothecin (HCPT) on oral squamous carcinoma cell line (Tca8113).
The cytotoxicity of HCPT on Tca8113 cell line was measured by MTT assay and cell cycle detected by flow cytometry(FCM). The growth status of Tca8113 cell xenografts following treatment with HCPT was observed. The doubling times and tumor inhibition rate were calculated.
HCPT had strong cytotoxicity on Tca8113 cells, the IC50 was 2 mumol/L. After treatment with low concentration of HCPT, the cell cycle was arrested in S phase or G2 + M phase; while at high concentration, apoptosis was obviously found and the rate of apoptosis was increased as the time and concentration of HCPT. Compared with control group, the xenografts of HCPT treated group grew slower and tumor doubling time was prolonged, The tumor volumes of HCPT treated group at 28th day were significantly smaller(P < 0.001) with tumor inhibition rate of 69.6%.
The result showed that HCPT had strong cytotoxicity effect to oral squamous carcinoma cells and significant growth inhibition on Tca8113 xenografts. HCPT can arrest cell cycle in S phase and G2 + M phase and induce cell apoptosis.
研究表明，羟基喜树碱（HCPT）对多种恶性肿瘤具有治疗作用，但关于口腔鳞状细胞癌的研究报道较少。本研究旨在探讨羟基喜树碱（HCPT）对口腔鳞状癌细胞系（Tca8113）的抗癌作用及机制。
采用MTT法检测HCPT对Tca8113细胞系的细胞毒性，应用流式细胞术（FCM）检测细胞周期。观察HCPT处理后Tca8113细胞异种移植瘤的生长状况。计算倍增时间和肿瘤抑制率。
HCPT对Tca8113细胞具有较强的细胞毒性，IC50为2μmol/L。低浓度HCPT处理后，细胞周期阻滞于S期或G2+M期；高浓度时，明显出现凋亡，且凋亡率随HCPT作用时间和浓度的增加而升高。与对照组相比，HCPT处理组异种移植瘤生长较慢，肿瘤倍增时间延长，第28天HCPT处理组肿瘤体积显著减小（P<0.001），肿瘤抑制率为69.6%。
结果表明，HCPT对口腔鳞状癌细胞具有较强的细胞毒性作用，对Tca8113异种移植瘤具有显著的生长抑制作用。HCPT可使细胞周期阻滞于S期和G2+M期，并诱导细胞凋亡。
