Zhao Min, Wu Xin-xing
Department of Molecular Biology, Medical College of Wuhan University, Wuhan 430071, P. R. China.
Ai Zheng. 2002 Jun;21(6):640-3.
BACKGROUND & OBJECTIVE: The infection with human papillomavirus and cellular genetic alteration appear to be necessary etiological factors for cervical carcinoma. This study was designed to investigate the genetic instability in cervical carcinoma tissues and provide evidence for discovering new tumor suppressor genes and screening diagnostic molecular marker of cervical carcinoma.
Fifty primary cervical carcinoma specimens from high-incidence area was examined through loss of heterozygosity(LOH) and microsatellite instability(MI) analysis with eight polymorphic microsatellite markers on chromosome 3, 6, 11, and 18.
LOH was observed at one or more loci in 33 cases(66%). The highest frequency of the allelic loss was found in D18s474 (18q21, 40.5%). LOH was detected on other sites including D3s1478 (3p21.3-21.2, 31.7%), D3s1766 (18q21.32, 15.0%), D6s260 (6p23, 23.3%), D11s925 (11q22-23, 17.9%), D18s35 (18q21.1-21.31, 8.7%), D18s64 (18q21.32, 16.7%), and D18s68 (18q22.1, 27.3%). MI was detected in 4 cases(8%).
Different percentages LOH on specific chromosomal regions were found and MI was very infrequent in cervical carcinoma tissues. The results of the current study strongly suggest that the putative suppressor gene(s) likely to be located on specific chromosome region 3p or 18q involve in cervical tumorigenesis.
人乳头瘤病毒感染及细胞基因改变似乎是宫颈癌必要的病因学因素。本研究旨在探讨宫颈癌组织中的基因不稳定性，为发现新的肿瘤抑制基因及筛选宫颈癌诊断分子标志物提供依据。
采用位于3号、6号、11号和18号染色体上的8个多态性微卫星标记，通过杂合性缺失（LOH）及微卫星不稳定性（MI）分析，检测了来自高发区的50例原发性宫颈癌标本。
33例（66%）在一个或多个位点观察到LOH。等位基因缺失频率最高的是D18s474（18q21，40.5%）。在其他位点也检测到LOH，包括D3s1478（3p21.3 - 21.2，31.7%）、D3s1766（18q21.32，15.0%）、D6s260（6p23，23.3%）、D11s925（11q22 - 23，17.9%）、D18s35（18q21.1 - 21.31，8.7%）、D18s64（18q21.32，16.7%）和D18s68（18q22.1，27.3%）。4例（8%）检测到MI。
在宫颈癌组织中发现特定染色体区域存在不同比例的LOH，且MI很少见。本研究结果强烈提示，可能位于特定染色体区域3p或18q的假定抑癌基因参与了宫颈癌的发生。
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