Kisseljov F, Semionova L, Samoylova E, Mazurenko N, Komissarova E, Zourbitskaya V, Gritzko T, Kozachenko V, Netchushkin M, Petrov S, Smirnov A, Alonso A
Department of Viral Molecular Biology, Kazan Cancer Research Center, Moscow, Russia.
Int J Cancer. 1996 Dec 20;69(6):484-7. doi: 10.1002/(SICI)1097-0215(19961220)69:6<484::AID-IJC12>3.0.CO;2-1.
Loss of heterozygosity (LOH) in chromosome 6 in human squamous cervical carcinomas was analyzed in the long and short arms of the chromosome using 3 pairs of primers each. In all cases, normal adjacent tissue was used as control. Among 51 cases analyzed, we identified LOH or microsatellite instability in 23% using primer D6S291 (located at position 6p21.3) and in 11% using primers D6S308 (6q16.3-6q27) and D6S270 (6q22.3-6q23.2). On the contrary, no significant LOH or genomic instabilities were detected with primers D6S306 (6p22.3-6p21.2), D6S299 (6p22.3-6p21.3) and D6S287 (6q21-6q23.3). Our results thus suggest the existence of instable loci at 3 regions of chromosome 6. Whether these loci contain putative tumor-suppressor genes or genes involved in cell cycle control remains unknown.
利用每对3对引物，对人类宫颈鳞状细胞癌6号染色体的长臂和短臂进行杂合性缺失（LOH）分析。所有病例均以相邻正常组织作为对照。在分析的51例病例中，使用引物D6S291（位于6p21.3位置）时，我们发现23%的病例存在LOH或微卫星不稳定性；使用引物D6S308（6q16.3 - 6q27）和D6S270（6q22.3 - 6q23.2）时，11%的病例存在上述情况。相反，使用引物D6S306（6p22.3 - 6p21.2）、D6S299（6p22.3 - 6p21.3）和D6S287（6q21 - 6q23.3）时，未检测到明显的LOH或基因组不稳定性。因此，我们的结果表明6号染色体的3个区域存在不稳定位点。这些位点是否包含假定的肿瘤抑制基因或参与细胞周期调控的基因仍不清楚。
