使用太赫兹技术的无标记生物亲和检测

Label-free bioaffinity detection using terahertz technology.

作者信息

Mickan Samuel P, Menikh Abdellah, Liu Haibo, Mannella Carmen A, MacColl Robert, Abbott Derek, Munch Jesper, Zhang X C

机构信息

Center for Terahertz Research, Department of Physics, Applied Physics & Astronomy, Rensselaer Polytechnic Institute, NY 12180, USA.

出版信息

Phys Med Biol. 2002 Nov 7;47(21):3789-95. doi: 10.1088/0031-9155/47/21/317.

DOI:10.1088/0031-9155/47/21/317

PMID:12452569

Abstract

We report the first use of differential terahertz time-domain spectroscopy for bioaffinity sensing. Binding is observed by measuring the transmission of a thin layer of biotin bound to the sensor protein avidin. We demonstrate the THz wave transmission of a sub-micron-thick film and sensitivity to 0.1 microg cm(-2) of biotin. These results point the way for a host of biosensor applications using T-rays, or pulsed far-infrared (FIR) radiation.

摘要

我们报道了首次将差分太赫兹时域光谱用于生物亲和传感。通过测量与传感器蛋白抗生物素蛋白结合的生物素薄层的透射率来观察结合情况。我们展示了亚微米厚薄膜的太赫兹波透射率以及对0.1微克/平方厘米生物素的灵敏度。这些结果为一系列使用太赫兹射线或脉冲远红外（FIR）辐射的生物传感器应用指明了方向。