Weisenstein Christian, Schaar Dominik, Katharina Wigger Anna, Schäfer-Eberwein Heiko, Bosserhoff Anja K, Haring Bolívar Peter
Institute of High Frequency and Quantum Electronics HQE, University of Siegen, Germany.
Institute of Biochemistry, Friedrich-Alexander-University Erlangen-Nürnberg, Germany.
Biomed Opt Express. 2019 Dec 23;11(1):448-460. doi: 10.1364/BOE.380818. eCollection 2020 Jan 1.
THz technologies are a powerful tool for label-free detection of biomolecules. However, significant reduction of the lower detection limit is required to apply THz-sensors in biomedical diagnosis. This paper reports an ultrasensitive THz-biosensor based on asymmetric double split ring resonators (aDSRR) for the direct label- and PCR-free detection of DNA at physiologically relevant concentrations. We introduce selective functionalization and localized electric field concentration to enhance aDSRR sensitivity and specificity. The sensor characteristics are demonstrated using the human tumor marker MIA in cDNA samples produced from total RNA without PCR-amplification. Measurements of DNA samples with concentrations as low as 1.55 × 10 mol/l are presented.
太赫兹技术是用于生物分子无标记检测的强大工具。然而，要将太赫兹传感器应用于生物医学诊断，需要大幅降低检测下限。本文报道了一种基于不对称双裂环谐振器（aDSRR）的超灵敏太赫兹生物传感器，用于在生理相关浓度下直接对DNA进行无标记和无PCR检测。我们引入了选择性功能化和局部电场集中来提高aDSRR的灵敏度和特异性。使用从总RNA产生的cDNA样本中的人类肿瘤标志物MIA，在不进行PCR扩增的情况下展示了传感器特性。给出了浓度低至1.55×10⁻⁹mol/l的DNA样本的测量结果。
