基于频率选择表面的用于无PCR cDNA检测的超灵敏太赫兹生物传感器。
Ultrasensitive THz biosensor for PCR-free cDNA detection based on frequency selective surfaces.
作者信息
Weisenstein Christian, Schaar Dominik, Katharina Wigger Anna, Schäfer-Eberwein Heiko, Bosserhoff Anja K, Haring Bolívar Peter
机构信息
Institute of High Frequency and Quantum Electronics HQE, University of Siegen, Germany.
Institute of Biochemistry, Friedrich-Alexander-University Erlangen-Nürnberg, Germany.
出版信息
Biomed Opt Express. 2019 Dec 23;11(1):448-460. doi: 10.1364/BOE.380818. eCollection 2020 Jan 1.
Abstract
THz technologies are a powerful tool for label-free detection of biomolecules. However, significant reduction of the lower detection limit is required to apply THz-sensors in biomedical diagnosis. This paper reports an ultrasensitive THz-biosensor based on asymmetric double split ring resonators (aDSRR) for the direct label- and PCR-free detection of DNA at physiologically relevant concentrations. We introduce selective functionalization and localized electric field concentration to enhance aDSRR sensitivity and specificity. The sensor characteristics are demonstrated using the human tumor marker MIA in cDNA samples produced from total RNA without PCR-amplification. Measurements of DNA samples with concentrations as low as 1.55 × 10 mol/l are presented.
摘要
太赫兹技术是用于生物分子无标记检测的强大工具。然而，要将太赫兹传感器应用于生物医学诊断，需要大幅降低检测下限。本文报道了一种基于不对称双裂环谐振器（aDSRR）的超灵敏太赫兹生物传感器，用于在生理相关浓度下直接对DNA进行无标记和无PCR检测。我们引入了选择性功能化和局部电场集中来提高aDSRR的灵敏度和特异性。使用从总RNA产生的cDNA样本中的人类肿瘤标志物MIA，在不进行PCR扩增的情况下展示了传感器特性。给出了浓度低至1.55×10⁻⁹mol/l的DNA样本的测量结果。
相似文献
1
Ultrasensitive THz biosensor for PCR-free cDNA detection based on frequency selective surfaces.基于频率选择表面的用于无PCR cDNA检测的超灵敏太赫兹生物传感器。
Biomed Opt Express. 2019 Dec 23;11(1):448-460. doi: 10.1364/BOE.380818. eCollection 2020 Jan 1.
2
Highly sensitive detection of malignant glioma cells using metamaterial-inspired THz biosensor based on electromagnetically induced transparency.基于电磁感应透明的超材料太赫兹生物传感器用于恶性神经胶质瘤细胞的高灵敏检测。
Biosens Bioelectron. 2021 Aug 1;185:113241. doi: 10.1016/j.bios.2021.113241. Epub 2021 Apr 22.
3
High specificity THz metamaterial-based biosensor for label-free transcription factor detection in melanoma diagnostics.基于太赫兹超材料的高特异性生物传感器，用于无标记黑色素瘤诊断中转录因子的检测。
Sci Rep. 2023 Nov 24;13(1):20708. doi: 10.1038/s41598-023-46876-5.
4
Ultrasensitive label-free detection of PNA-DNA hybridization by reduced graphene oxide field-effect transistor biosensor.基于还原氧化石墨烯场效应晶体管生物传感器的超灵敏无标记 PNA-DNA 杂交检测。
ACS Nano. 2014 Mar 25;8(3):2632-8. doi: 10.1021/nn4063424. Epub 2014 Feb 20.
5
Nano metamaterials for ultrasensitive Terahertz biosensing.用于超灵敏太赫兹生物传感的纳米超材料。
Sci Rep. 2017 Aug 15;7(1):8146. doi: 10.1038/s41598-017-08508-7.
6
An aptamer biosensor for leukemia marker mRNA detection based on polymerase-assisted signal amplification and aggregation of illuminator.基于聚合酶辅助信号放大和照明剂聚集的白血病标志物 mRNA 检测适体生物传感器。
Anal Bioanal Chem. 2019 Jan;411(1):139-146. doi: 10.1007/s00216-018-1424-9. Epub 2018 Oct 29.
7
Aptamer-based microcantilever biosensor for ultrasensitive detection of tumor marker nucleolin.基于适配体的微悬臂梁生物传感器用于超灵敏检测肿瘤标志物核仁素。
Talanta. 2016 Jan 1;146:727-31. doi: 10.1016/j.talanta.2015.06.034. Epub 2015 Jun 18.
8
An ultrasensitive label free human papilloma virus DNA biosensor using gold nanotubes based on nanoporous polycarbonate in electrical alignment.一种基于纳米多孔聚碳酸酯中电排列的金纳米管的超灵敏无标记人乳头瘤病毒DNA生物传感器。
Anal Chim Acta. 2019 Feb 7;1048:31-41. doi: 10.1016/j.aca.2018.09.062. Epub 2018 Sep 28.
9
Integrated planar terahertz resonators for femtomolar sensitivity label-free detection of DNA hybridization.用于飞摩尔灵敏度无标记检测DNA杂交的集成平面太赫兹谐振器。
Appl Opt. 2002 Apr 1;41(10):2074-8. doi: 10.1364/ao.41.002074.
10
A Microbolometer System for Radiation Detection in the THz Frequency Range with a Resonating Cavity Fabricated in the CMOS Technology.一种用于太赫兹频率范围辐射检测的微测辐射热计系统，其带有采用CMOS技术制造的谐振腔。
Recent Pat Nanotechnol. 2018 Feb 14;12(1):34-44. doi: 10.2174/1872210511666170704103627.
引用本文的文献
1
Strong coupling of metamaterials with cavity photons: toward non-Hermitian optics.超材料与腔光子的强耦合：迈向非厄米光学。
Nanophotonics. 2024 Feb 5;13(14):2443-2451. doi: 10.1515/nanoph-2023-0899. eCollection 2024 Jun.
2
AI-Based Metamaterial Design.基于人工智能的超材料设计。
ACS Appl Mater Interfaces. 2024 Jun 12;16(23):29547-29569. doi: 10.1021/acsami.4c04486. Epub 2024 May 29.
3
A CMOS-integrated terahertz near-field sensor based on an ultra-strongly coupled meta-atom.一种基于超强耦合元原子的互补金属氧化物半导体集成太赫兹近场传感器。
Sci Rep. 2024 May 20;14(1):11483. doi: 10.1038/s41598-024-61971-x.
4
High specificity THz metamaterial-based biosensor for label-free transcription factor detection in melanoma diagnostics.基于太赫兹超材料的高特异性生物传感器，用于无标记黑色素瘤诊断中转录因子的检测。
Sci Rep. 2023 Nov 24;13(1):20708. doi: 10.1038/s41598-023-46876-5.
5
Terahertz Imaging and Spectroscopy in Cancer Diagnostics: A Technical Review.太赫兹成像与光谱技术在癌症诊断中的应用：技术综述
BME Front. 2020 Sep 25;2020:2547609. doi: 10.34133/2020/2547609. eCollection 2020.
6
Multifrequency Investigation of Single- and Double-Stranded DNA with Scalable Metamaterial-Based THz Biosensors.基于可扩展超材料太赫兹生物传感器的单链和双链 DNA 的多频研究。
Biosensors (Basel). 2022 Jul 1;12(7):483. doi: 10.3390/bios12070483.
7
Terahertz thermal curve analysis for label-free identification of pathogens.太赫兹热曲线分析用于无标记的病原体识别。
Nat Commun. 2022 Jun 16;13(1):3470. doi: 10.1038/s41467-022-31137-2.
本文引用的文献
1
Quantitative characterization of bovine serum albumin thin-films using terahertz spectroscopy and machine learning methods.利用太赫兹光谱和机器学习方法对牛血清白蛋白薄膜进行定量表征。
Biomed Opt Express. 2018 Jun 6;9(7):2917-2929. doi: 10.1364/BOE.9.002917. eCollection 2018 Jul 1.
2
A Route to Terahertz Metamaterial Biosensor Integrated with Microfluidics for Liver Cancer Biomarker Testing in Early Stage.太赫兹超材料生物传感器与微流控集成用于早期肝癌生物标志物检测的途径。
Sci Rep. 2017 Nov 27;7(1):16378. doi: 10.1038/s41598-017-16762-y.
3
On-Chip THz Detection of Biomaterials: A Numerical Study.生物材料的片上太赫兹检测：一项数值研究。
J Biol Phys. 2003 Jun;29(2-3):187-94. doi: 10.1023/A:1024444809852.
4
Terahertz-time domain spectroscopy for the detection of PCR amplified DNA in aqueous solution.太赫兹时域光谱法在水溶液中 PCR 扩增 DNA 的检测。
Analyst. 2012 Feb 7;137(3):575-9. doi: 10.1039/c2an15820e. Epub 2011 Dec 21.
5
Sharp trapped-mode resonances in planar metamaterials with a broken structural symmetry.具有结构对称性破缺的平面超材料中的尖锐俘获模共振。
Phys Rev Lett. 2007 Oct 5;99(14):147401. doi: 10.1103/PhysRevLett.99.147401. Epub 2007 Oct 2.
6
Self-assembled monolayers of thiolates on metals as a form of nanotechnology.金属上硫醇盐的自组装单分子层作为一种纳米技术形式。
Chem Rev. 2005 Apr;105(4):1103-69. doi: 10.1021/cr0300789.
7
Babinet principle applied to the design of metasurfaces and metamaterials.巴比涅原理在超表面和超材料设计中的应用。
Phys Rev Lett. 2004 Nov 5;93(19):197401. doi: 10.1103/PhysRevLett.93.197401. Epub 2004 Nov 1.
8
Self-contained, fully integrated biochip for sample preparation, polymerase chain reaction amplification, and DNA microarray detection.用于样品制备、聚合酶链反应扩增和DNA微阵列检测的独立式、全集成生物芯片。
Anal Chem. 2004 Apr 1;76(7):1824-31. doi: 10.1021/ac0353029.
9
A functionalized THz sensor for marker-free DNA analysis.一种用于无标记DNA分析的功能化太赫兹传感器。
Phys Med Biol. 2003 Nov 21;48(22):3625-36. doi: 10.1088/0031-9155/48/22/001.
10
Far-infrared vibrational modes of DNA components studied by terahertz time-domain spectroscopy.用太赫兹时域光谱研究DNA组分的远红外振动模式。
Phys Med Biol. 2002 Nov 7;47(21):3807-14. doi: 10.1088/0031-9155/47/21/319.
Zotero 插件