体外评估致敏和未致敏人淋巴细胞的放射敏感性。
Radiosensitivity of sensitized and nonsensitized human lymphocytes evaluated in vitro.
作者信息
Nichols W S, Troup G M, Anderson R E
出版信息
Am J Pathol. 1975 Jun;79(3):499-508.
The purpose of this study is to compare the relative radiosensitivity of sensitized and nonsensitized lymphocytes utilizing an in vitro test system. The test system employed is the combined mixed leukocyte reaction and cell-mediated lympholysis assays (MLC-CML). In this system, sensitized T lymphocytes of human origin are much more resistant to radiation-induced injury than are nonsensitized lymphocytes. The results support similar observation in vivo with antigen-activated lymphocytes and are interpreted in one of two ways: a) sensitized T cell, rendered nonviable by radiation, are still able to exert lytic activity, perhaps via the release of cytotoxic factors; and b) sensitized T cells are inherently radioresistant, perhaps due to the hypermetabolic state of such cells, which may serve to activate repair mechanisms.
本研究的目的是利用体外测试系统比较致敏淋巴细胞和未致敏淋巴细胞的相对放射敏感性。所采用的测试系统是混合白细胞反应和细胞介导的淋巴细胞溶解试验（MLC-CML）相结合。在该系统中，人源致敏T淋巴细胞比未致敏淋巴细胞对辐射诱导的损伤具有更强的抵抗力。这些结果支持了在体内对抗原激活淋巴细胞的类似观察，并可以通过以下两种方式之一进行解释：a）因辐射而失去活力的致敏T细胞仍能够发挥溶解活性，可能是通过释放细胞毒性因子；b）致敏T细胞本身具有抗辐射能力，可能是由于此类细胞的高代谢状态，这可能有助于激活修复机制。
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