Parsons Y M, Shaw K L
Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Genetics. 2002 Nov;162(3):1275-82. doi: 10.1093/genetics/162.3.1275.
As with many organisms of evolutionary interest, the Hawaiian cricket Laupala genome is not well characterized genetically. Mapping such an unexplored genome therefore presents challenges not often faced in model genetic organisms and not well covered in the literature. We discuss the evolutionary merits of Laupala as a model for speciation studies involving prezygotic change, our choice of marker system for detecting genetic variation, and the initial genetic expectations pertaining to the construction of any unknown genomic map in general and to the Laupala linkage map construction in particular. We used the technique of amplified fragment length polymorphism (AFLP) to develop a linkage map of Laupala. We utilized both EcoRI/MseI- and EcoRI/PstI-digested genomic DNA to generate AFLP bands and identified 309 markers that segregated among F(2) interspecific hybrid individuals. The map is composed of 231 markers distributed over 11 and 7 species-specific autosomal groups together with a number of putative X chromosome linkage groups. The integration of codominant markers enabled the identification of five homologous linkage groups corresponding to five of the seven autosomal chromosomal pairs found in Laupala.
与许多具有进化研究价值的生物一样，夏威夷蟋蟀Laupala的基因组在遗传学上尚未得到充分表征。因此，绘制这样一个未被探索的基因组面临着模型遗传生物中不常遇到且文献中未充分涵盖的挑战。我们讨论了将Laupala作为涉及合子前变化的物种形成研究模型的进化优势、我们用于检测遗传变异的标记系统的选择，以及一般而言构建任何未知基因组图谱特别是构建Laupala连锁图谱的初始遗传预期。我们使用扩增片段长度多态性（AFLP）技术来构建Laupala的连锁图谱。我们利用经EcoRI/MseI和EcoRI/PstI消化的基因组DNA来产生AFLP条带，并鉴定了309个在F(2)种间杂交个体中分离的标记。该图谱由分布在11个和7个物种特异性常染色体组以及一些假定的X染色体连锁组上的231个标记组成。共显性标记的整合使得能够鉴定出与Laupala中发现的七个常染色体对中的五个相对应的五个同源连锁组。
