Lusardi Theresa A, Smith Douglas H, Wolf John A, Meaney David F
Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104-6392, USA.
Biorheology. 2003;40(1-3):401-9.
In this study, we examine the response of cultured hippocampal neurons to mechanical stretch. To measure the immediate response, we measured the response of a calcium sensitive fluorescent dye (Fura-2AM) prior to and for five minutes following stretch. In separate experiments, we measured the viability of the cells using propidium iodide labeling at 24 hours following a single stretch. Results show that cytosolic calcium increases immediately following stretch to levels much higher than those associated with maximal chemical agonist (NMDA) stimulation. Moreover, the calcium response data indicate a loss in calcium regulation in the neurons at the most severe level of stretch. Finally, at the highest levels of stretch, the cell survivability decreases at 24 hrs following the stretch injury. These data show the association between loss in calcium regulation and early signs of neuronal cell death.
在本研究中，我们检测了培养的海马神经元对机械拉伸的反应。为了测量即时反应，我们在拉伸前以及拉伸后五分钟测量了钙敏感荧光染料（Fura-2AM）的反应。在单独的实验中，我们在单次拉伸后24小时使用碘化丙啶标记来测量细胞活力。结果显示，拉伸后胞质钙立即增加，达到远高于与最大化学激动剂（NMDA）刺激相关的水平。此外，钙反应数据表明，在最严重的拉伸水平下，神经元的钙调节功能丧失。最后，在最高拉伸水平下，拉伸损伤后24小时细胞存活率降低。这些数据显示了钙调节功能丧失与神经元细胞死亡早期迹象之间的关联。
