Kimura A, Hirose K, Kariya Y, Nagai S
J Bacteriol. 1976 Feb;125(2):744-6. doi: 10.1128/jb.125.2.744-746.1976.
Respiration-deficient mutants (Rho-, petite) of Saccharomyces carlsbergensis were obtained by treatment with trypaflavin (euflavine). Dried cells of these mutants phosphorylated mononucleotides to their triphosphates and further formed not only cytidine 5'-diphosphate-choline, but also sugar nucleotides, such as uridine 5'-diphosphate-glucose, guanosine 5'-diphosphate-mannose, etc. The activities were the same or slightly greater than those of the wild strain. These results showed that energy (adenosine 5'-triphosphate) necessary for phosphorylation of mononucleotides was sufficiently supplied by the glycolysis system.
通过用锥黄素（依佛黄素）处理，获得了卡尔斯伯酵母的呼吸缺陷型突变体（Rho-，小菌落）。这些突变体的干燥细胞将单核苷酸磷酸化为其三磷酸形式，不仅进一步形成了胞苷5'-二磷酸胆碱，还形成了糖核苷酸，如尿苷5'-二磷酸葡萄糖、鸟苷5'-二磷酸甘露糖等。这些活性与野生菌株相同或略高。这些结果表明，单核苷酸磷酸化所需的能量（腺苷5'-三磷酸）由糖酵解系统充分提供。
