Nagai S
Biological Laboratories, National Women's University, Nara, Japan.
J Bacteriol. 1965 Jul;90(1):220-2. doi: 10.1128/jb.90.1.220-222.1965.
Nagai, Susumu (National Women's University, Nara, Japan). Differential reduction of tellurite by growing colonies of normal yeast and respiration-deficient mutants. J. Bacteriol. 90:220-222. 1965.-A differential reduction of sodium tellurite was observed between normal and respiration-deficient mutant colonies of several species of Saccharomyces. Normal colonies turned black in contrast to mutant colonies which remained nearly white when grown on an agar medium containing 30 to 40 mg per liter of tellurite. Schopfer's medium enriched with yeast extract and a mixture of vitamins was most suitable to develop such black-and-white contrast. The difference was far less obvious when the asparagine of this medium was replaced by other nitrogen sources such as glutamate, peptone, or Casamino Acids. Addition of ammonium sulfate to the medium weakened and sometimes completely reversed the contrast. The usefulness of tellurite medium for diagnostic color differentiation of respiration deficiency was considered.
长井进（日本奈良女子大学）。正常酵母和呼吸缺陷型突变体生长菌落对亚碲酸盐的差异还原。《细菌学杂志》90:220 - 222。1965年。——在几种酿酒酵母的正常菌落和呼吸缺陷型突变体菌落之间观察到亚碲酸钠的差异还原。当在每升含有30至40毫克亚碲酸盐的琼脂培养基上生长时，正常菌落变黑，而突变体菌落几乎保持白色。富含酵母提取物和维生素混合物的朔普费尔培养基最适合形成这种黑白对比。当该培养基中的天冬酰胺被其他氮源如谷氨酸、蛋白胨或酪蛋白氨基酸取代时，差异远不那么明显。向培养基中添加硫酸铵会减弱并有时完全逆转这种对比。考虑了亚碲酸盐培养基在呼吸缺陷诊断颜色区分中的用途。
