Brown J H, Walker S, Casto L, Howell R R
J Chromatogr. 1976 Jan 21;116(2):293-304. doi: 10.1016/s0021-9673(00)89900-8.
A Fortran IV computer program is described which identifies, computes, and statistically evaluates amino acid concentrations determined by an automatic amino acid analyzer in physiologic fluids. The program accepts retention time and intergrated peak areas from two calibration standards for identification and computational reference. Two internal standards are used to compensate for variations in injector performance and ninhydrin decay. Calibration standard responses are statistically treated to assist in detection of equipment malfunction. The statistical data base is automatically explanded through inclusion of data from normal patients. Amino acid concentrations are printed with appropriate mean and standard deviation values in a format acceptable as a final report.
本文描述了一个Fortran IV计算机程序,该程序可识别、计算并统计评估由自动氨基酸分析仪测定的生理体液中的氨基酸浓度。该程序接受来自两种校准标准品的保留时间和积分峰面积,用于识别和计算参考。使用两种内标来补偿进样器性能变化和茚三酮衰减。对校准标准品的响应进行统计处理,以协助检测设备故障。通过纳入正常患者的数据,统计数据库会自动扩展。氨基酸浓度会以适当的平均值和标准差数值打印出来,其格式可作为最终报告接受。