通过高压冷冻对盘基网柄菌中的肌动蛋白杆和束进行电子显微镜观察。

Electron microscopy of actin rods and bundles in Dictyostelium discoideum by high-pressure freezing.

作者信息

Sameshima Masazumi, Kishi Yoshiro, Osumi Masako, Mahadeo Dana, Cotter David A

出版信息

J Electron Microsc (Tokyo). 2002;51(5):337-40. doi: 10.1093/jmicro/51.5.337.

DOI:10.1093/jmicro/51.5.337

Abstract

A new type of actin rods comprising actin tubules appears in dormant spores of Dictyostelium discoideum. Occasionally, the rods in the nucleus were observed in an amorphous state using a combination of high-pressure freezing and freeze-substitutions. Also in the case of actin bundles formed in the nudeus of vegetative cells exposed to dimethyl sulphoxide, actin filaments seemed to be embedded in matrices. The karyoplasm of spores fixed by the above method appeared to be denser than that obtained by other methods. As soluble materials may be efficiently retained in the nucleus, actin tubules or actin filaments embedded in those materials may result in hazy images of actin rods and bundles.

摘要

一种由肌动蛋白微管组成的新型肌动蛋白棒出现在盘基网柄菌的休眠孢子中。偶尔，使用高压冷冻和冷冻置换相结合的方法观察到细胞核中的棒呈无定形状态。同样，在暴露于二甲基亚砜的营养细胞的细胞核中形成肌动蛋白束的情况下，肌动蛋白丝似乎嵌入基质中。通过上述方法固定的孢子的核质似乎比通过其他方法获得的核质更致密。由于可溶性物质可能有效地保留在细胞核中，嵌入这些物质中的肌动蛋白微管或肌动蛋白丝可能导致肌动蛋白棒和束的模糊图像。