二甲基亚砜诱导核肌动蛋白束的动力学及影响其形成的因素
Dynamics of nuclear actin bundle induction by dimethyl sulfoxide and factors affecting its development.
作者信息
Fukui Y, Katsumaru H
出版信息
J Cell Biol. 1980 Jan;84(1):131-40. doi: 10.1083/jcb.84.1.131.
Abstract
We have reported in a previous paper that dimethyl sulfoxide (DMSO) induces the formation of huge bundles of actin filaments in the nuclei of Dictyostelium mucoroides. The present study was performed to provide electron microscope data on the induction of nuclear actin bundles, illustrating both their formation and their reversion, as well as on the effects of various factors on the induction. The large nuclear bundles of actin appeared after 20--30 min of treatment with 10% DMSO. A DMSO concentration of 5 or 10% was optimal for the induction of the bundles. The nuclear actin bundle reverted to the original morphology within 5 min after removing DMSO. Induction of nuclear actin bundles was inhibited by Mg++ and low temperatures, but not by Na+, K+, Ca++, ATP, 3'5'-cyclic adenosine monophosphate (cAMP), phosphate buffer, or cytochalasin B. Neither NaN3 nor cycloheximide totally inhibited the induction of the bundles.
摘要
我们在之前的一篇论文中报道过，二甲基亚砜（DMSO）可诱导黏液霉状网柄菌细胞核中形成巨大的肌动蛋白丝束。本研究旨在提供有关核肌动蛋白束诱导的电子显微镜数据，阐明其形成和逆转过程，以及各种因素对诱导的影响。用10% DMSO处理20 - 30分钟后出现大型核肌动蛋白束。5%或10%的DMSO浓度对束的诱导最为适宜。去除DMSO后5分钟内，核肌动蛋白束恢复到原始形态。Mg++和低温可抑制核肌动蛋白束的诱导，但Na+、K+、Ca++、ATP、3',5'-环磷酸腺苷（cAMP）、磷酸盐缓冲液或细胞松弛素B则无此作用。NaN3和环己酰亚胺均不能完全抑制束的诱导。
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