ALEX (artificial liver for extracorporeal xenoassistance): a new bioreactor containing a porcine autologous biomatrix as hepatocyte support. Preliminary results in an ex vivo experimental model.
作者信息
Ambrosino G, Varotto S, Basso S, Galavotti D, Cecchetto A, Carraro P, Naso A, De Silvestro G, Plebani M, Giron G, Abatangelo G, Donato D, Braga G P, Cestrone A, Marrelli L, Trombetta M, Lorenzelli V, Picardi A, Valente M L, Palu G, Colantoni A, Van Thiel D, Ricordi C, D'Amico D F
机构信息
Department of Surgical and Gastroenterological Sciences, Section of General and Liver Transplant Surgery, University of Padova School of Medicine, Padova, Italy.
出版信息
Int J Artif Organs. 2002 Oct;25(10):960-5. doi: 10.1177/039139880202501010.
Long-term maintenance of viability and expression of differentiated hepatocyte function is crucial for bioartificial liver support. We developed a new bioreactor design (ALEX), associated with a new extracellular autologous hepatocyte biomatrix (Porcine Autologous Biomatrix - PBM) support. To test this new bioreactor, we compared it to a standard BAL (BioArtificial Liver) cartridge in a ex vivo model using human plasma added to bilirubin, ammonium and lidocaine. A pathology study was performed on both bioreactors. The results suggest that ALEX allows a maximal contact between the perfusing plasma and the liver cells and a proper hepatocyte support by a cell-to-matrix attachment. ALEX is a suitable cell support bioreactor, guaranteeing long-term maintenance of the metabolic activity of hepatocytes when compared to a standard BAL cartridge.
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