Chen Zhong, Ding Yi-Tao
Department of General Surgery, Affiliated Hospital, Nantong University, Nantong 226001, Jiangsu Province, China.
World J Gastroenterol. 2006 Feb 28;12(8):1312-6. doi: 10.3748/wjg.v12.i8.1857.
To evaluate the functions of a new bioartificial liver (BAL) system in vitro and in vitro.
The BAL system was configured by inoculating porcine hepatocyte spheroids into the cell circuit of a hollow fiber bioreactor. In the experiments of BAL in vitro, the levels of alanine aminotransferase (ALT), total bilirubin (TB), and albumin (ALB) in the circulating hepatocyte suspension and RPMI-1640 medium were determined during 6 h of circulation in the BAL device. In the experiments of BAL in vitro, acute liver failure (ALF) model in canine was induced by an end-side portocaval shunt combined with common bile duct ligation and transaction. Blood ALT, TB and ammonia levels of ALF in canines were determined before and after BAL treatment.
During 6 h of circulation in vitro, there was no significant change of ALT, whereas the TB and ALB levels gradually increased with time both in the hepatocyte suspension and in RPMI-1640 medium. In the BAL treatment group, blood ALT, TB and ammonia levels of ALF in canines decreased significantly.
The new BAL system has the ability to perform liver functions and can be used to treat ALF.
评估一种新型生物人工肝（BAL）系统的体内外功能。
通过将猪肝细胞球接种到中空纤维生物反应器的细胞回路中来构建BAL系统。在BAL体外实验中，在BAL装置中循环6小时期间，测定循环肝细胞悬液和RPMI-1640培养基中丙氨酸转氨酶（ALT）、总胆红素（TB）和白蛋白（ALB）的水平。在BAL体内实验中，通过端侧门腔分流联合胆总管结扎和切断诱导犬急性肝衰竭（ALF）模型。测定BAL治疗前后犬ALF的血ALT、TB和氨水平。
在体外循环6小时期间，ALT无明显变化，而肝细胞悬液和RPMI-1640培养基中的TB和ALB水平均随时间逐渐升高。在BAL治疗组中，犬ALF的血ALT、TB和氨水平显著降低。
新型BAL系统具有执行肝功能的能力，可用于治疗ALF。
