Shimamoto K, Murase T, Yamaji T
J Lab Clin Med. 1976 Feb;87(2):338-44.
A sensitive and specific radioimmunoassay for arginine vasopressin (AVP) was developed utilizing the antisera against lysine vasopressin (LVP) in combination with a labeled AVP. The assay employs an acetone extraction procedure and detects as little as 0.8 pg. per milliliter of AVP in human plasma. In normal subjects the mean (+/- S.D.) plasma concentration of AVP was 4.9 +/- 1.2 pg. per mililiter after fluid deprivation and 1.2 +/- 0.4 pg. per milliliter after water loading. Plasma AVP levels correlated significantly with plasma osmolalities. In four patients with diabetes insipidus, plasma AVP concentrations ranged from less than 0.8 to 1.2 pg. per milliliter, whereas six patients with the syndrome of inappropriate ADH secretion showed plasma levels of AVP which correspond to those of the dehydrated state in normal subjects or greater, although plasma osmolalities were low in all cases. It was concluded that the present radioimmunoassay method for AVP provides a useful way of assessing neurohypophyseal function in man.
利用抗赖氨酸加压素（LVP）的抗血清与标记的精氨酸加压素（AVP）相结合，开发了一种灵敏且特异的抗利尿激素放射免疫测定法。该测定法采用丙酮提取程序，可检测出人血浆中低至每毫升0.8皮克的抗利尿激素。在正常受试者中，禁水后抗利尿激素的平均（±标准差）血浆浓度为每毫升4.9±1.2皮克，饮水后为每毫升1.2±0.4皮克。血浆抗利尿激素水平与血浆渗透压显著相关。在4例尿崩症患者中，血浆抗利尿激素浓度范围为每毫升低于0.8至1.2皮克，而6例抗利尿激素分泌不当综合征患者的血浆抗利尿激素水平与正常受试者脱水状态时相当或更高，尽管所有病例的血浆渗透压均较低。得出的结论是，目前的抗利尿激素放射免疫测定方法为评估人类神经垂体功能提供了一种有用的方法。
