Poh-Fitzpatrick M B, Lamola A A
J Lab Clin Med. 1976 Feb;87(2):362-70.
Primary and secondary porphyrinemias are characterized by the presence of several porphyrin species in erythrocytes and/or plasma. Acid solvent extraction of porphyrins from natural ligands can preclude observation of significant spectral differences apparent on direct spectrofluorometric examination of porphyrin complexes in tissue. Erythrocytes and hemoglobin-free plasma obtained from 13 patients with erythropoietic, 9 with lead intoxication, 5 with porphyria cutanea tarda, 3 with iron deficiency anemia, 1 with erythropoietic porphyria, and 10 normal persons were simply diluted and analyzed spectrofluorometrically. Characteristic porphyrin-ligand spectral patterns were consistently obtained for each group of specimens, allowing diagnostic application of the data.
原发性和继发性卟啉血症的特征是红细胞和/或血浆中存在多种卟啉种类。从天然配体中酸溶剂提取卟啉可能会妨碍在组织中对卟啉复合物进行直接荧光光谱检查时观察到明显的光谱差异。从13例红细胞生成性患者、9例铅中毒患者、5例迟发性皮肤卟啉病患者、3例缺铁性贫血患者、1例红细胞生成性卟啉病患者和10名正常人获取的红细胞和无血红蛋白血浆被简单稀释后进行荧光光谱分析。每组标本均一致获得了特征性的卟啉-配体光谱模式，从而使这些数据可用于诊断。
