恒河猴体外血脑屏障的特性研究
Characterization of an in vitro rhesus macaque blood-brain barrier.
作者信息
MacLean Andrew G, Orandle Marlene S, MacKey John, Williams Kenneth C, Alvarez Xavier, Lackner Andrew A
机构信息
New England Regional Primate Research Center, Harvard Medical School, Southborough, MA 01772-9102, USA.
出版信息
J Neuroimmunol. 2002 Oct;131(1-2):98-103. doi: 10.1016/s0165-5728(02)00256-4.
Abstract
The blood-brain barrier (BBB) has been modeled in vitro in a number of species, including rat, cow and human. Coculture of multiple cell types is required for the correct expression of tight junction proteins by microvascular brain endothelial cells (MBEC). Markers of inflammation, especially MHC-II, and cell adhesion molecules, such as VCAM-1, are not expressed on the luminal surface of the barrier under resting conditions. The rhesus macaque model has been used to study early events of HIV-neuropathogenesis in vivo, but a suitable in vitro model has not been available for detailed mechanistic studies. Here we describe an in vitro rhesus macaque blood-brain barrier that utilizes autologous MBEC and astrocytes. We believe that this model is highly relevant for examining immunological events at the blood-brain barrier and demonstrate its potential usefulness for examining early events in AIDS neuropathogenesis.
摘要
血脑屏障（BBB）已在包括大鼠、牛和人类在内的多种物种中进行了体外建模。微血管脑内皮细胞（MBEC）正确表达紧密连接蛋白需要多种细胞类型的共培养。在静息条件下，炎症标志物，尤其是MHC-II，以及细胞粘附分子，如VCAM-1，不会在屏障的管腔表面表达。恒河猴模型已被用于研究体内HIV神经发病机制的早期事件，但尚未有适合进行详细机制研究的体外模型。在此，我们描述了一种利用自体MBEC和星形胶质细胞的体外恒河猴血脑屏障。我们认为该模型与检查血脑屏障处的免疫事件高度相关，并证明了其在检查艾滋病神经发病机制早期事件方面的潜在用途。
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