Chow Christine S, Cunningham Philip R, Lee KangSeok, Meroueh May, SantaLucia John, Varma Shikha
Department of Chemistry, Wayne State University, Detroit, MI 48202, USA.
Biochimie. 2002 Sep;84(9):859-68. doi: 10.1016/s0300-9084(02)01403-7.
Photoinduced cleavage reactions by the rhodium complex tris(4,7-diphenyl-1,10-phenanthroline)rhodium(III) [Rh(DIP)(3)(3+)] with three RNA hairpins, r(GGGGU UCGCUC CACCA) (16 nucleotide, tetraloop(Ala2)), r(GGGGCUAUAGCUCUAGCUC CACCA) (24 nucleotide, microhelix(Ala)), and r(GGCGGUUAGAUAUCGCC) (17 nucleotide, 790 loop), and full-length (1542 nucleotide) 16S rRNA from Escherichia coli were investigated. The cleavage reactions were monitored by gel electrophoresis and the sites of cleavage by Rh(DIP)(3)(3+) were determined by comparisons with chemical or enzymatic sequencing reactions. In general, RNA backbone scission by the metal complex was induced at G.U mismatches and at exposed G residues. The cleavage activity was observed on the three small RNA hairpins as well as on the isolated 1542-nucleotide ribosomal RNA.
研究了铑配合物三（4,7-二苯基-1,10-菲咯啉）铑（III）[Rh（DIP）(3)(3+)]与三个RNA发夹结构r（GGGGU UCGCUC CACCA）（16个核苷酸，四环（Ala2））、r（GGGG CUAUAG CUCUAG CUC CACCA）（24个核苷酸，微螺旋（Ala））和r（GGCGGUUAGAUAUCGCC）（17个核苷酸，790环）以及来自大肠杆菌的全长（1542个核苷酸）16S rRNA的光诱导切割反应。通过凝胶电泳监测切割反应，并通过与化学或酶促测序反应比较来确定Rh（DIP）(3)(3+)的切割位点。一般来说，金属配合物诱导的RNA主链断裂发生在G.U错配处和暴露的G残基处。在三个小RNA发夹结构以及分离的1542核苷酸核糖体RNA上均观察到了切割活性。
