铑配合物在大小RNA中的G·U错配和暴露的鸟嘌呤处引发光诱导切割。

Photoinduced cleavage by a rhodium complex at G.U mismatches and exposed guanines in large and small RNAs.

作者信息

Chow Christine S, Cunningham Philip R, Lee KangSeok, Meroueh May, SantaLucia John, Varma Shikha

机构信息

Department of Chemistry, Wayne State University, Detroit, MI 48202, USA.

出版信息

Biochimie. 2002 Sep;84(9):859-68. doi: 10.1016/s0300-9084(02)01403-7.

DOI:10.1016/s0300-9084(02)01403-7

PMID:12458078

Abstract

Photoinduced cleavage reactions by the rhodium complex tris(4,7-diphenyl-1,10-phenanthroline)rhodium(III) [Rh(DIP)(3)(3+)] with three RNA hairpins, r(GGGGU UCGCUC CACCA) (16 nucleotide, tetraloop(Ala2)), r(GGGGCUAUAGCUCUAGCUC CACCA) (24 nucleotide, microhelix(Ala)), and r(GGCGGUUAGAUAUCGCC) (17 nucleotide, 790 loop), and full-length (1542 nucleotide) 16S rRNA from Escherichia coli were investigated. The cleavage reactions were monitored by gel electrophoresis and the sites of cleavage by Rh(DIP)(3)(3+) were determined by comparisons with chemical or enzymatic sequencing reactions. In general, RNA backbone scission by the metal complex was induced at G.U mismatches and at exposed G residues. The cleavage activity was observed on the three small RNA hairpins as well as on the isolated 1542-nucleotide ribosomal RNA.

摘要

研究了铑配合物三（4,7-二苯基-1,10-菲咯啉）铑（III）[Rh（DIP）(3)(3+)]与三个RNA发夹结构r（GGGGU UCGCUC CACCA）（16个核苷酸，四环（Ala2））、r（GGGG CUAUAG CUCUAG CUC CACCA）（24个核苷酸，微螺旋（Ala））和r（GGCGGUUAGAUAUCGCC）（17个核苷酸，790环）以及来自大肠杆菌的全长（1542个核苷酸）16S rRNA的光诱导切割反应。通过凝胶电泳监测切割反应，并通过与化学或酶促测序反应比较来确定Rh（DIP）(3)(3+)的切割位点。一般来说，金属配合物诱导的RNA主链断裂发生在G.U错配处和暴露的G残基处。在三个小RNA发夹结构以及分离的1542核苷酸核糖体RNA上均观察到了切割活性。