前列腺质膜的制备 大鼠腹侧前列腺中（Na⁺，K⁺）-ATP酶和Mg²⁺-ATP酶的分布
Preparation of prostatic plasma membranes Distribution of (Na-+, K-+)-ATPase and Mg-2+-ATPase in the rat ventral prostate.
作者信息
Wilson M J, Villee C A
出版信息
Biochim Biophys Acta. 1975 Jun 11;394(1):1-9. doi: 10.1016/0005-2736(75)90200-x.
PMID:124595
Abstract
- A method is described for the isolation of a plasma membrane fraction from rat ventral prostate. This fraction is greatly enriched in (Na-", K-+)-ATPase but also contains a small amount of Mg-2+-ATPase and 5'-nucleotidase activities. 2. The activity ratio (Mg-2+ plus Na-+ plus K-+/Mg-2+ or Mg-+ plus Na-+) of the plasma membrane (Na-+, K-+)-ATPase was 1.7. The enzyme system specifically requires ATP as substrate and is inhibited by Ca-2+ or ouabain.
摘要
- 描述了一种从大鼠腹侧前列腺中分离质膜组分的方法。该组分中（Na⁺, K⁺）-ATP酶大量富集，但也含有少量Mg²⁺-ATP酶和5'-核苷酸酶活性。2. 质膜（Na⁺, K⁺）-ATP酶的活性比率（Mg²⁺ + Na⁺ + K⁺/Mg²⁺ 或Mg²⁺ + Na⁺）为1.7。该酶系统特异性地需要ATP作为底物，并受到Ca²⁺或哇巴因的抑制。
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