Rajendhran J, Krishnakumar V, Gunasekaran P
Department of Microbial Technology, Centre for Advanced Studies in Functional Genomics, School of Biological Sciences, Madurai Kamaraj University, Madurai, India.
Lett Appl Microbiol. 2002;35(6):523-7. doi: 10.1046/j.1472-765x.2002.01234.x.
Optimization of Penicillin G acylase (PAC) production from a novel isolate of Bacillus sp.
Fermentation medium for PAC production was optimized using a two-level fractional factorial design with seven components.
A maximum production of 9.5 U ml(-1) of PAC was obtained in an optimized medium containing (g l(-1): K2HPO4, 1.0; MgSO4.7H2O, 0.1; CaCl2.2H2O, 0.1; PAA, 2.0; tryptone, 5.0; yeast extract, 3.0; and sucrose, 50.0.
The two-step medium optimization resulted in a twofold increase in PAC production. Since the strain Bacillus sp. PGS10 produces a high level of PAC, it could be a potential candidate for industrial production of PAC.
对芽孢杆菌属新分离菌株生产青霉素G酰化酶（PAC）进行优化。
采用含七个成分的二级分式析因设计对生产PAC的发酵培养基进行优化。
在优化培养基中（g l(-1)）：磷酸氢二钾1.0；七水硫酸镁0.1；二水氯化钙0.1；聚丙烯酸2.0；胰蛋白胨5.0；酵母提取物3.0；蔗糖50.0，获得了最高产量为9.5 U ml(-1)的PAC。
两步培养基优化使PAC产量提高了两倍。由于芽孢杆菌属菌株PGS10能产生高水平的PAC，它可能是PAC工业生产的潜在候选菌株。
