温度、柠檬酸铁铵和甘氨酸甜菜碱之间的相互作用对单核细胞增生李斯特菌在限定培养基中生长的影响
Influence of interactions between temperature, ferric ammonium citrate and glycine betaine on the growth of Listeria monocytogenes in a defined medium.
作者信息
Dykes G A, Dworaczek M
机构信息
Department of Applied Microbiology and Food Science, University of Saskatchewan, 51 Campus Drive, Saskatoon SK S7N 5A8, Canada.
出版信息
Lett Appl Microbiol. 2002;35(6):538-42. doi: 10.1046/j.1472-765x.2002.01237.x.
AIMS
To investigate interactions, if any, between temperature, ferric ammonium citrate and glycine betaine on the growth of Listeria monocytogenes in modified Pine's medium (Pine et al. 1989).
METHODS AND RESULTS
Modified Pine's medium containing 0, 0.044, 0.088 or 0.176 g l(-1) ferric ammonium citrate, and 0 or 1 mM glycine betaine, was inoculated with each of two L. monocytogenes strains and incubated at 4, 25 or 37 degrees C. The optical density at 600 nm, and cell numbers, were determined at appropriate time intervals. At 4 degrees C, but not other temperatures, increasing ferric ammonium citrate resulted in improved growth in the absence, but not the presence, of glycine betaine. The presence of glycine betaine was inhibitory at 25 and 37 degrees C, but not at 4 degrees C.
CONCLUSIONS
Interactions affecting the growth kinetics of L. monocytogenes were apparent between the parameters investigated.
SIGNIFICANCE AND IMPACT OF THE STUDY
Limitations on the use of modified Pine's medium, and the significance of iron metabolism at lower temperatures, were revealed.
目的
研究在改良的派恩培养基（Pine等人，1989年）中，温度、柠檬酸铁铵和甘氨酸甜菜碱之间是否存在相互作用，若存在则研究其对单核细胞增生李斯特菌生长的影响。
方法与结果
用两种单核细胞增生李斯特菌菌株分别接种含有0、0.044、0.088或0.176 g l(-1)柠檬酸铁铵以及0或1 mM甘氨酸甜菜碱的改良派恩培养基，并在4、25或37℃下培养。在适当的时间间隔测定600 nm处的光密度和细胞数量。在4℃时，而非其他温度下，在不存在甘氨酸甜菜碱时，增加柠檬酸铁铵会促进生长，但在存在甘氨酸甜菜碱时则不然。甘氨酸甜菜碱的存在在25和37℃时具有抑制作用，但在4℃时没有。
结论
在所研究的参数之间，影响单核细胞增生李斯特菌生长动力学的相互作用是明显的。
研究的意义与影响
揭示了改良派恩培养基使用的局限性以及低温下铁代谢的重要性。
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