El-Rifai Wa'el, Moskaluk Christopher A, Abdrabbo Mohammad Khalouck, Harper Jeffery, Yoshida Cynthia, Riggins Gregory J, Frierson Henry F, Powell Steven M
Department of Medicine, University of Virginia Health System, Charlottesville, Virginia 22908-0708, USA.
Cancer Res. 2002 Dec 1;62(23):6823-6.
Serial analysis of gene expression provides quantitative and comprehensive expression profiling in a given cell population. In our efforts to define the genes overexpressed in carcinoma of the stomach, we performed serial analysis of gene expression analyses on dissected neoplastic and normal gastric epithelia. We identified 91,334 expressed tags, including 26,633 that were unique. The 20 most up-regulated genes (P < 0.01) in gastric cancer (GC) compared with normal gastric epithelia included several keratins that are specific for epithelial cells such as keratin 6A, 13, and 17. Interestingly, five calcium-binding proteins (S100A2, S100A7, S100A8, S100A9, and S100A10) were overexpressed. Quantitative real-time PCR on primary GC samples demonstrated overexpression of S100A2 in 18 of 20 tumors (90%). The other calcium-binding proteins were overexpressed in 25-45% of the GC samples that we studied. Our results indicate that S100A proteins may be important for gastric tumorigenesis. Additional investigations are required to elucidate the biological role of calcium-binding proteins in cancer.
基因表达序列分析可在特定细胞群体中提供定量且全面的表达谱。为了确定在胃癌中过表达的基因，我们对分离出的肿瘤性和正常胃上皮进行了基因表达序列分析。我们鉴定出91,334个表达标签，其中包括26,633个独特标签。与正常胃上皮相比，胃癌（GC）中上调最明显的20个基因（P < 0.01）包括几种上皮细胞特异性角蛋白，如角蛋白6A、13和17。有趣的是，5种钙结合蛋白（S100A2、S100A7、S100A8、S100A9和S100A10）过表达。对原发性GC样本进行的定量实时PCR显示，20个肿瘤中有18个（90%）的S100A2过表达。我们研究的其他钙结合蛋白在25% - 45%的GC样本中过表达。我们的结果表明，S100A蛋白可能对胃癌发生很重要。需要进一步研究以阐明钙结合蛋白在癌症中的生物学作用。
