Mouyna I, Sarfati J, Recco P, Fontaine T, Henrissatz B, Latge J P
Institut Pasteur, Unité des Aspergillus, 25 rue du Docteur Roux 75724 Paris Cedex 15, France.
Med Mycol. 2002 Oct;40(5):455-64. doi: 10.1080/mmy.40.5.455.464.
A 74 kDa beta(1-3)endoglucanase of Aspergillus fumigatus was recently isolated from a cell wall autolysate and biochemically characterized. In this study, we report the cloning and the disruption of the ENGL1 gene encoding this beta(1-3)endoglucanase. ENGL1 contains an open reading frame of 2181 bp encoding a polypeptide of 727 amino acids. Sequence analysis showed that ENGL1 is the first characterized member of a new family of beta(1-3)glucanases. Disruption of ENGL1, however, did not lead to a phenotype distinct from the parental strain, indicating that this cell wall-associated beta(1-3)endoglucanase does not play an essential role in constitutive cell growth.
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