Choi Jinhee, Caquet Thierry, Roche Hélène
Laboratory of Ecology and Zoology, University of Paris-Sud, CNRS UPRESA 8079, Bât. 442, F 91405, Orsay, France.
Environ Toxicol Chem. 2002 Dec;21(12):2725-30.
The effects of fenitrothion exposure on fourth-instar Chimnomus riparius larvae were investigated on biochemical, physiological, and population-level parameters. Biochemical effects were investigated through measurements of acetylcholinesterase and cytosolic superoxide dismutase activities. Water content and dry weight of the larvae were used as physiological parameters, and the emergence rate of adults was used as a descriptor of population-level effects. Results showed that the response of most parameters exhibited a concentration-dependent relationship. Although biochemical parameters proved to be very sensitive, no direct relation was observed with effects at a higher level of biological organization. Perturbations of osmoregulation, as reflected by changes in water content of the larvae, were more directly related with emergence failure. This study demonstrates that the use of several biological parameters can provide complementary information about the effects of chemical exposure. Therefore, use of a multilevel approach in C. riparius seems to be a promising way to diagnose environmental quality.
研究了杀螟硫磷暴露对四龄河岸摇蚊幼虫在生化、生理和种群水平参数方面的影响。通过测量乙酰胆碱酯酶和胞质超氧化物歧化酶活性来研究生化影响。将幼虫的含水量和干重作为生理参数，成虫羽化率作为种群水平影响的描述指标。结果表明，大多数参数的响应呈现浓度依赖性关系。尽管生化参数被证明非常敏感，但未观察到与更高生物组织水平效应的直接关系。幼虫含水量变化所反映的渗透调节紊乱与羽化失败更直接相关。本研究表明，使用多个生物学参数可以提供有关化学物质暴露影响的补充信息。因此，在河岸摇蚊中采用多水平方法似乎是诊断环境质量的一种有前途的方法。
