Nakagawa Yuki, Saito Kazuhide, Morioka Testuo, Tomita Yoshihiko, Takahashi Kota, Oite Takashi
Department of Cellular Physiology, Institute of Nephrology, Graduate School of Medical and Dental Sciences, Niigata University, Japan.
Clin Transplant. 2002;16 Suppl 8:51-7. doi: 10.1034/j.1399-0012.16.s8.10.x.
Vascular endothelial cells (ECs) are considered to be a primary target for injury in allograft rejection. However, the relationship between serum antibody activity to ECs and rejection episodes has not been examined extensively in renal transplantation. Twenty-two renal transplant recipients were included in this study. Serum antibody activity to vascular endothelial cells (AECA) was measured using a cellular enzyme-linked immunosorbent assay (ELISA) in which human umbilical vein endothelial cells (HUVEC) and human glomerular endothelial cells (HGEC) were preincubated with TNF-alpha used as target cells. Serum samples were obtained just before transplantation and once a week during the immediate 1-3-month post-transplantation period. There was a significant correlation between the presence of AECA against HGEC and rejection episodes (P < 0.05). Patients with multi-episodes of rejection showed significantly higher frequencies of AECA than patients with mono-episodic rejection (P < 0.0005). It should be noted that patients suffering from multi-episodes of rejection revealed higher AECA titres before transplantation. These findings imply that the HGEC-ELISA could be used as a prospective, informative test to identify patients with a higher risk of acute rejection in renal transplantation.
血管内皮细胞（ECs）被认为是同种异体移植排斥反应中损伤的主要靶点。然而，在肾移植中，血清抗内皮细胞抗体活性与排斥反应发作之间的关系尚未得到广泛研究。本研究纳入了22名肾移植受者。采用细胞酶联免疫吸附测定（ELISA）法检测血清抗血管内皮细胞抗体（AECA）活性，该方法以人脐静脉内皮细胞（HUVEC）和人肾小球内皮细胞（HGEC）作为靶细胞，并预先用肿瘤坏死因子-α（TNF-α）进行孵育。在移植前及移植后1 - 3个月内每周采集一次血清样本。抗HGEC的AECA的存在与排斥反应发作之间存在显著相关性（P < 0.05）。多次发生排斥反应的患者AECA频率显著高于单次发作排斥反应的患者（P < 0.0005）。需要注意的是，多次发生排斥反应的患者在移植前AECA滴度更高。这些发现表明，HGEC-ELISA可作为一种前瞻性、有信息量的检测方法，用于识别肾移植中急性排斥反应风险较高的患者。
