性腺甾体激素对大鼠纹状体中谷氨酸受体表达的调节作用。

Modulation of glutamate receptor expression by gonadal steroid hormones in the rat striatum.

作者信息

D'Souza Deborah N, Harlan Richard E, Garcia Meredith M

机构信息

Department of Structural and Cellular Biology, Tulane University Medical School, New Orleans, LA 70112, USA.

出版信息

Brain Res Bull. 2003 Jan 15;59(4):289-92. doi: 10.1016/s0361-9230(02)00885-7.

DOI:10.1016/s0361-9230(02)00885-7

PMID:12464400

Abstract

The non-competitive N-methyl-d-aspartate (NMDA) receptor antagonist dizocilpine (MK-801) attenuates morphine-induced immediate-early gene expression in the rat striatum in a sexually dimorphic manner that depends in part on gonadal steroids. To determine if this effect was dependent on modulation of glutamate receptor gene expression, we studied the effect of gonadal hormones on levels of the NR1 subunit of NMDA receptor and the GluR2 subunit of the AMPA-subtype of glutamate receptor in the rat striatum, using autoradiographic immunocytochemistry. We found that ovariectomy decreased GluR2 immunoreactivity in the striatum, but no changes were seen in levels of NR1 following gonadectomy in either sex. Thus, the effects of gonadal steroids on NMDA receptor-mediated responses are not due to regulation of NR1 expression.

摘要

非竞争性N-甲基-D-天冬氨酸（NMDA）受体拮抗剂地佐环平（MK-801）能以一种性别差异的方式减弱吗啡诱导的大鼠纹状体即刻早期基因表达，这种性别差异部分取决于性腺类固醇。为了确定这种效应是否依赖于谷氨酸受体基因表达的调节，我们使用放射自显影免疫细胞化学方法，研究了性腺激素对大鼠纹状体中NMDA受体的NR1亚基和谷氨酸受体AMPA亚型的GluR2亚基水平的影响。我们发现，卵巢切除降低了纹状体中GluR2的免疫反应性，但两性性腺切除后NR1水平均未发生变化。因此，性腺类固醇对NMDA受体介导反应的影响并非由于NR1表达的调节。