Chobotova Katya, Spyropoulou Isabella, Carver Janet, Manek Sanjiv, Heath John K, Gullick William J, Barlow David H, Sargent Ian L, Mardon Helen J
Nuffield Department of Obstetrics and Gynaecology, The Women's Centre, Level 3, John Radcliffe Hospital, University of Oxford, Headington, Oxford, OX3 9DU, UK.
Mech Dev. 2002 Dec;119(2):137-44. doi: 10.1016/s0925-4773(02)00342-8.
The mechanisms that mediate implantation of the human embryo remain poorly understood and represent a fundamental problem in reproductive biology. Candidate molecules that mediate and facilitate implantation have been identified in animal studies, and include heparin binding epidermal growth factor. Here we demonstrate a potential function for the transmembrane form of heparin-binding epidermal growth factor in mediating blastocyst attachment to the endometrium, in two different novel in vitro models for human implantation. Furthermore, we demonstrate specific localisation of the heparin-binding epidermal growth factor receptor ErbB4, on the surface of the trophectoderm in peri-implantation human blastocysts. Our data lead the way for further dissection of the molecular mechanisms of implantation of the human embryo, and have implications for infertility, in vitro fertilization and contraception.
介导人类胚胎着床的机制仍未得到充分理解，是生殖生物学中的一个基本问题。在动物研究中已鉴定出介导和促进着床的候选分子，包括肝素结合表皮生长因子。在此，我们在两种不同的新型人类着床体外模型中，证明了肝素结合表皮生长因子跨膜形式在介导囊胚附着于子宫内膜方面的潜在功能。此外，我们证明了肝素结合表皮生长因子受体ErbB4在着床期人类囊胚滋养外胚层表面的特异性定位。我们的数据为进一步剖析人类胚胎着床的分子机制开辟了道路，并对不孕症、体外受精和避孕具有重要意义。
