HIV复制的RNA干扰

RNA interference of HIV replication.

作者信息

Martínez Miguel Angel, Clotet Bonaventura, Esté José A

机构信息

Retrovirology Laboratory IrsiCaixa, Hospital Universitari Germans Trias i Pujol, Universitat Autònoma de Barcelona, 08916 Badalona, Spain.

出版信息

Trends Immunol. 2002 Dec;23(12):559-61. doi: 10.1016/s1471-4906(02)02328-1.

DOI:10.1016/s1471-4906(02)02328-1

PMID:12464559

Abstract

Double-stranded RNA-mediated interference (RNAi) induces sequence-specific post-transcriptional gene silencing and has emerged as a powerful tool to silence gene expression in multiple organisms. In mammalian cells, duplexes of 21 nucleotide RNAs, known as short-interfering RNAs (siRNAs), efficiently inhibit gene expression. Recent research demonstrates the general use of siRNAs to specifically inhibit HIV-1 replication by targeting viral or cellular genes. Importantly, RNAi opens a new avenue for gene-based therapeutics.

摘要

双链RNA介导的干扰（RNAi）可诱导序列特异性的转录后基因沉默，并已成为在多种生物体中沉默基因表达的强大工具。在哺乳动物细胞中，21个核苷酸的RNA双链体，即小干扰RNA（siRNA），能有效抑制基因表达。最近的研究表明，siRNA可通过靶向病毒或细胞基因来普遍用于特异性抑制HIV-1复制。重要的是，RNAi为基于基因的治疗开辟了一条新途径。