通过RNA干扰实现病毒复制的基因沉默

Gene silencing of virus replication by RNA interference.

作者信息

Miyano-Kurosaki N, Takaku H

机构信息

Department of Life and Environmental Sciences and High Technology Research Center, Chiba Institute of Technology, 2-17-1 Narashino, Tsudanuma, 275-0016 Chiba, Japan.

出版信息

Handb Exp Pharmacol. 2006(173):151-71. doi: 10.1007/3-540-27262-3_8.

DOI:10.1007/3-540-27262-3_8

PMID:16594615

Abstract

Small interfering RNAs (siRNAs) are as effective as long double-stranded RNAs (dsRNAs) at targeting and silencing genes by RNA interference (RNAi). siRNAs are widely used for assessing gene function in cultured mammalian cells or early developing vertebrate embryos. They are also promising reagents for developing gene-specific therapeutics. The specific inhibition of viral replication is particularly well suited to RNAi, as several stages of the viral life cycle and many viral and cellular genes can be targeted. The future success of this approach will depend on the recent advances in siRNA-based clinical trials.

摘要

小干扰RNA（siRNA）在通过RNA干扰（RNAi）靶向和沉默基因方面与长双链RNA（dsRNA）一样有效。siRNA广泛用于评估培养的哺乳动物细胞或早期发育的脊椎动物胚胎中的基因功能。它们也是开发基因特异性疗法的有前景的试剂。病毒复制的特异性抑制特别适合RNAi，因为病毒生命周期的几个阶段以及许多病毒和细胞基因都可以成为靶点。这种方法未来的成功将取决于基于siRNA的临床试验的最新进展。