Kawano Shin, Tajima Kenji, Uemori Yukako, Yamashita Hitomi, Erata Tomoki, Munekata Masanobu, Takai Mitsuo
Division of Molecular Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo 060-8628, Japan.
DNA Res. 2002 Oct 31;9(5):149-56. doi: 10.1093/dnares/9.5.149.
About 14.5 kb of DNA fragments from Acetobacter xylinum ATCC23769 and ATCC53582 were cloned, and their nucleotide sequences were determined. The sequenced DNA regions contained endo-beta-1,4-glucanase, cellulose complementing protein, cellulose synthase subunit AB, C, D and beta-glucosidase genes. The results from a homology search of deduced amino acid sequences between A. xylinum ATCC23769 and ATCC53582 showed that they were highly similar. However, the amount of cellulose production by ATCC53582 was 5 times larger than that of ATCC23769 during a 7-day incubation. In A. xylinum ATCC53582, synthesis of cellulose continued after glucose was consumed, suggesting that a metabolite of glucose, or a component of the medium other than glucose, may be a substrate of cellulose. On the other hand, cell growth of ATCC23769 was twice that of ATCC53582. Glucose is the energy source in A. xylinum as well as the substrate of cellulose synthesis, and the metabolic pathway of glucose in both strains may be different. These results suggest that the synthesis of cellulose and the growth of bacterial cells are contradictory.
从木醋杆菌ATCC23769和ATCC53582中克隆了约14.5 kb的DNA片段，并测定了它们的核苷酸序列。测序的DNA区域包含内切β-1,4-葡聚糖酶、纤维素互补蛋白、纤维素合酶亚基AB、C、D和β-葡萄糖苷酶基因。木醋杆菌ATCC23769和ATCC53582之间推导氨基酸序列的同源性搜索结果表明它们高度相似。然而，在7天的培养期间，ATCC53582的纤维素产量是ATCC23769的5倍。在木醋杆菌ATCC53582中，葡萄糖消耗后纤维素合成仍在继续，这表明葡萄糖的代谢产物或培养基中除葡萄糖以外的成分可能是纤维素的底物。另一方面，ATCC23769的细胞生长是ATCC53582的两倍。葡萄糖既是木醋杆菌的能量来源，也是纤维素合成的底物，两种菌株中葡萄糖的代谢途径可能不同。这些结果表明纤维素的合成与细菌细胞的生长是相互矛盾的。
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