利用遗传互补方法阐明细菌核糖核酸酶P蛋白亚基的结构-功能关系。
Elucidation of structure-function relationships in the protein subunit of bacterial RNase P using a genetic complementation approach.
作者信息
Jovanovic Milan, Sanchez Ruth, Altman Sidney, Gopalan Venkat
机构信息
Department of Biochemistry, The Ohio State University, Columbus, OH 43210, USA.
出版信息
Nucleic Acids Res. 2002 Dec 1;30(23):5065-73. doi: 10.1093/nar/gkf670.
Abstract
RNase P is a ribonucleoprotein involved in tRNA biosynthesis in all living organisms. Bacterial RNase P is comprised of a catalytic RNA subunit and a lone protein cofactor which plays a supporting, albeit essential, role in the tRNA processing reaction in vivo. In this study, we have searched various databases to identify homologs of the protein subunit of RNase P from diverse bacteria and used an alignment of their primary sequences to determine the most highly conserved residues, and thereby extend earlier predictions of which residues might play an important role in RNA recognition. By employing a genetic complementation assay, we have also gained insights into structure- function relationships in the protein subunit of bacterial RNase P.
摘要
核糖核酸酶P是一种核糖核蛋白，参与所有生物体的转运RNA生物合成。细菌核糖核酸酶P由一个催化性RNA亚基和一个单独的蛋白质辅因子组成，该辅因子在体内转运RNA加工反应中发挥支持作用，尽管这一作用至关重要。在本研究中，我们搜索了各种数据库，以鉴定来自不同细菌的核糖核酸酶P蛋白质亚基的同源物，并利用它们一级序列的比对来确定最保守的残基，从而扩展了对哪些残基可能在RNA识别中发挥重要作用的早期预测。通过采用遗传互补分析，我们还深入了解了细菌核糖核酸酶P蛋白质亚基的结构-功能关系。
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本文引用的文献
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