新鲜分离的人小肠上皮细胞对白细胞介素-1α、白细胞介素-1β和白细胞介素-6的差异表达及上调

Differential expression and upregulation of interleukin-1alpha, interleukin-1beta and interleukin-6 by freshly isolated human small intestinal epithelial cells.

作者信息

Madrigal-Estebas Laura, Doherty Derek G, O'Donoghue Diarmuid P, Feighery Conleth, O'Farrelly Cliona

机构信息

Education and Research Centre, St Vincent's University Hospital, Dublin, Ireland.

出版信息

Mediators Inflamm. 2002 Oct;11(5):313-9. doi: 10.1080/09629350210000015728.

DOI:10.1080/09629350210000015728

PMID:12467524

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1781673/

Abstract

BACKGROUND

Small intestinal epithelial cells (SIEC) may contribute to local immune regulation.

AIM

To examine production of interleukin (IL)-1alpha, IL-1beta and IL-6 by freshly isolated human SIEC.

METHODS

IL-1alpha and IL-1beta mRNA in epithelial layers (EL) prepared from small intestine and in intestinal epithelial cell (EC) lines were examined by reverse transcription-polymerase chain reaction. IL-1alpha, IL-1beta and IL-6 protein expression by SIEC was examined by flow cytometry before and after activation with lipopolysaccharide and epithelial growth factor.

RESULTS

IL-1alpha and IL-1beta mRNA was detected in EL and EC lines. Background expression of IL-1alpha and IL-1beta protein by SIEC was observed, which did not increase even following activation. IL-6 protein was expressed by SIEC, in a proportion that increased in two out of three samples following activation.

CONCLUSIONS

IL-6 expression and the presence of IL-1alpha and IL-1beta mRNA suggest a role for SIEC in the regulation of local inflammation.

摘要

背景

小肠上皮细胞（SIEC）可能参与局部免疫调节。

目的

检测新鲜分离的人SIEC产生白细胞介素（IL）-1α、IL-1β和IL-6的情况。

方法

采用逆转录-聚合酶链反应检测从小肠制备的上皮层（EL）和肠上皮细胞（EC）系中IL-1α和IL-1β mRNA。在用脂多糖和上皮生长因子激活前后，通过流式细胞术检测SIEC中IL-1α、IL-1β和IL-6蛋白的表达。

结果

在EL和EC系中检测到IL-1α和IL-1β mRNA。观察到SIEC对IL-1α和IL-1β蛋白的基础表达，即使在激活后也未增加。SIEC表达IL-6蛋白，在三个样本中的两个样本激活后其表达比例增加。

结论

IL-6的表达以及IL-1α和IL-1β mRNA的存在表明SIEC在局部炎症调节中发挥作用。

相似文献

Differential expression and upregulation of interleukin-1alpha, interleukin-1beta and interleukin-6 by freshly isolated human small intestinal epithelial cells.

Mediators Inflamm. 2002 Oct;11(5):313-9. doi: 10.1080/09629350210000015728.

PAR-2 activation in intestinal epithelial cells potentiates interleukin-1beta-induced chemokine secretion via MAP kinase signaling pathways.

Cytokine. 2005 Sep 7;31(5):358-67. doi: 10.1016/j.cyto.2005.06.004.

Interleukin 1 system and sex steroid receptor expression in human breast cancer: interleukin 1alpha protein secretion is correlated with malignant phenotype.

Clin Cancer Res. 2003 Oct 15;9(13):4877-83.

Macrophage migration inhibitory factor up-regulates the expression of interleukin-8 messenger RNA in synovial fibroblasts of rheumatoid arthritis patients: common transcriptional regulatory mechanism between interleukin-8 and interleukin-1beta.

Arthritis Rheum. 2004 May;50(5):1437-47. doi: 10.1002/art.20190.

Possible role of interleukin-1 in the regulation of bovine corpus luteum throughout the luteal phase.

Biol Reprod. 2004 Nov;71(5):1688-93. doi: 10.1095/biolreprod.104.032151. Epub 2004 Jul 14.

Effect of pituitary adenylate cyclase-activating polypeptide (PACAP) on IL-6, IL-8, and MCP-1 expression in human retinal pigment epithelial cell line.

Curr Eye Res. 2005 Dec;30(12):1105-11. doi: 10.1080/02713680500421444.

Interleukin-1alpha, 6 regulate the secretion of vascular endothelial growth factor A, C in pancreatic cancer.

Hepatobiliary Pancreat Dis Int. 2005 Aug;4(3):460-3.

Rapid expression of IL-1beta by intestinal epithelial cells in vitro.

Cell Immunol. 1999 Apr 10;193(1):1-8. doi: 10.1006/cimm.1999.1468.

Up-regulation of cyclooxygenase-2 by interleukin-1beta in colon carcinoma cells.

Cell Signal. 2006 Aug;18(8):1262-9. doi: 10.1016/j.cellsig.2005.10.009. Epub 2005 Dec 2.

Thrombin and PAR-1-AP increase proinflammatory cytokine expression in C6 cells.

J Surg Res. 2005 Dec;129(2):196-201. doi: 10.1016/j.jss.2005.07.041. Epub 2005 Sep 6.

引用本文的文献

Ethanolic Extract of Leaves Influences NF-κB Signaling Pathway to Restore Kidney Tissue from Cobalt-Mediated Oxidative Injury and Inflammation in Rats.

Nutrients. 2020 Apr 9;12(4):1031. doi: 10.3390/nu12041031.

An investigation of immunomodulatory properties of and cells and their extracellular polysaccharides.

Biosci Microbiota Food Health. 2017;36(3):101-110. doi: 10.12938/bmfh.17-001. Epub 2017 Mar 22.

In vitro evaluation of immunological properties of extracellular polysaccharides produced by Lactobacillus delbrueckii strains.

Biosci Microbiota Food Health. 2015;34(1):11-23. doi: 10.12938/bmfh.2014-013. Epub 2014 Nov 8.

Exogenous interleukin-6 facilitated the contraction of the colon in a depression rat model.

Dig Dis Sci. 2013 Aug;58(8):2187-96. doi: 10.1007/s10620-013-2656-3. Epub 2013 Apr 16.

本文引用的文献

Inhibition of Th1 differentiation by IL-6 is mediated by SOCS1.

Immunity. 2000 Dec;13(6):805-15. doi: 10.1016/s1074-7613(00)00078-9.

Lymphocyte CC chemokine receptor 9 and epithelial thymus-expressed chemokine (TECK) expression distinguish the small intestinal immune compartment: Epithelial expression of tissue-specific chemokines as an organizing principle in regional immunity.

J Exp Med. 2000 Sep 4;192(5):761-8. doi: 10.1084/jem.192.5.761.

Blockade of interleukin 6 trans signaling suppresses T-cell resistance against apoptosis in chronic intestinal inflammation: evidence in crohn disease and experimental colitis in vivo.

Nat Med. 2000 May;6(5):583-8. doi: 10.1038/75068.

IL-6 is required for the development of Th1 cell-mediated murine colitis.

J Immunol. 2000 May 1;164(9):4878-82. doi: 10.4049/jimmunol.164.9.4878.

Anti-inflammatory cytokines.

Chest. 2000 Apr;117(4):1162-72. doi: 10.1378/chest.117.4.1162.

Human intestinal epithelial cells secrete interleukin-1 receptor antagonist and interleukin-8 but not interleukin-1 or interleukin-6.

Gut. 2000 Mar;46(3):350-8. doi: 10.1136/gut.46.3.350.

Interleukin-6 and soluble interleukin-6 receptor in the colonic mucosa of inflammatory bowel disease.

J Gastroenterol Hepatol. 1999 Oct;14(10):987-96. doi: 10.1046/j.1440-1746.1999.01989.x.

Differing patterns of transforming growth factor-beta expression in normal intestinal mucosa and in active celiac disease.

J Pediatr Gastroenterol Nutr. 1999 Sep;29(3):308-13. doi: 10.1097/00005176-199909000-00013.

The human liver contains multiple populations of NK cells, T cells, and CD3+CD56+ natural T cells with distinct cytotoxic activities and Th1, Th2, and Th0 cytokine secretion patterns.

J Immunol. 1999 Aug 15;163(4):2314-21.

Interleukin 1 and interleukin 1beta converting enzyme (caspase 1) expression in the human colonic epithelial barrier. Caspase 1 downregulation in colon cancer.

Gut. 1999 Aug;45(2):246-51. doi: 10.1136/gut.45.2.246.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验

新鲜分离的人小肠上皮细胞对白细胞介素-1α、白细胞介素-1β和白细胞介素-6的差异表达及上调

Differential expression and upregulation of interleukin-1alpha, interleukin-1beta and interleukin-6 by freshly isolated human small intestinal epithelial cells.

作者信息

Madrigal-Estebas Laura, Doherty Derek G, O'Donoghue Diarmuid P, Feighery Conleth, O'Farrelly Cliona

机构信息

Education and Research Centre, St Vincent's University Hospital, Dublin, Ireland.

出版信息

Mediators Inflamm. 2002 Oct;11(5):313-9. doi: 10.1080/09629350210000015728.

DOI:10.1080/09629350210000015728

PMID:12467524

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1781673/

Abstract

BACKGROUND

Small intestinal epithelial cells (SIEC) may contribute to local immune regulation.

AIM

To examine production of interleukin (IL)-1alpha, IL-1beta and IL-6 by freshly isolated human SIEC.

METHODS

RESULTS

CONCLUSIONS

IL-6 expression and the presence of IL-1alpha and IL-1beta mRNA suggest a role for SIEC in the regulation of local inflammation.

摘要

背景

小肠上皮细胞（SIEC）可能参与局部免疫调节。

目的

检测新鲜分离的人SIEC产生白细胞介素（IL）-1α、IL-1β和IL-6的情况。

方法

结果

结论

IL-6的表达以及IL-1α和IL-1β mRNA的存在表明SIEC在局部炎症调节中发挥作用。

新鲜分离的人小肠上皮细胞对白细胞介素-1α、白细胞介素-1β和白细胞介素-6的差异表达及上调

Differential expression and upregulation of interleukin-1alpha, interleukin-1beta and interleukin-6 by freshly isolated human small intestinal epithelial cells.

作者信息

机构信息

出版信息

BACKGROUND

AIM

METHODS

RESULTS

CONCLUSIONS

背景

目的

方法

结果

结论

相似文献

引用本文的文献

本文引用的文献

文献AI研究员

用中文搜PubMed

文档翻译

Suppr 超能文献

新鲜分离的人小肠上皮细胞对白细胞介素-1α、白细胞介素-1β和白细胞介素-6的差异表达及上调

Differential expression and upregulation of interleukin-1alpha, interleukin-1beta and interleukin-6 by freshly isolated human small intestinal epithelial cells.

作者信息

机构信息

出版信息

BACKGROUND

AIM

METHODS

RESULTS

CONCLUSIONS

背景

目的

方法

结果

结论